http://www.cnr.it/ontology/cnr/individuo/prodotto/ID186149
LAMIN A/C AND N-MYC INTERPLAY IN NEUROBLASTOMA CELL DIFFERENTIATION (Contributo in atti di convegno)
- Type
- Label
- LAMIN A/C AND N-MYC INTERPLAY IN NEUROBLASTOMA CELL DIFFERENTIATION (Contributo in atti di convegno) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Alternative label
Maresca G., Felsani A., D'Agnano I (2010)
LAMIN A/C AND N-MYC INTERPLAY IN NEUROBLASTOMA CELL DIFFERENTIATION
in 27th National Conference of the Italian-Society-of-Cytometry-GIC, Ferrara, OCT 14-17, 2009
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- Maresca G., Felsani A., D'Agnano I (literal)
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- ISI Web of Science (WOS) (literal)
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- Istituto di Neurobiologia e Medicina Molecolare, CNR, Rome, Italy (literal)
- Titolo
- LAMIN A/C AND N-MYC INTERPLAY IN NEUROBLASTOMA CELL DIFFERENTIATION (literal)
- Abstract
- Neuroblastoma is a childhood tumor of the peripheral
sympathetic nervous system thought to arise from highly proliferative
migratory cells of the neural crest. Amplification of
the MYCN gene occurs in a number of neuroblastomas representing
a hallmark of a highly aggressive subgroup. Most malignant
neuroblastoma cells have retained their capacity to differentiate
in vitro. The type-V intermediate-filament lamins A/C
have an expression pattern in some organs dependent on the
differentiation states. In adult tissues, lamin A/C is observed
only in final differentiated cells. Our aim was to study the interplay
between MYCN and LMNA genes in the neuronal differentiation.
We employed the neuroblastoma cell lines SHSY5Y,
with high lamin A/C and absent N-Myc expression, and LAN-5,
with high N-Myc and low lamin A/C. Both cell lines were
induced to differentiate by retinoic acid. Differentiated
SHSY5Y cells reduced c-Myc protein expression, as expected
and increased lamin A/C, suggesting an involvement of the
lamins in the differentiation process. To better understand the
role of lamin A/C in the SHSY5Y differentiation we silenced
the LMNA gene. The decrease of lamin A/C determined an
inhibition of the neurites formation, even though a decrease of
the c-Myc protein was still evidenced. We then inhibited c-Myc
protein activity by using a validated peptide which interferes
with the Myc-Max dimerization. An increase of the neurofilament
and of lamin A/C expressions was observed after exposure
to the peptide, further indicating that lamin A/C is needed
to differentiate SHSY5Y cells. By contrast, LAN-5 cells significantly
increased N-Myc during their differentiation, while no
increase of lamin A/C was observed, strongly indicating an
interplay between NMYC and LMNA genes. (literal)
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