http://www.cnr.it/ontology/cnr/individuo/prodotto/ID186089
The Dof protein DAG1 mediates PIL5 activity on seed germination by negatively regulating the GA biosynthetic gene AtGA3ox1 (Articolo in rivista)
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- The Dof protein DAG1 mediates PIL5 activity on seed germination by negatively regulating the GA biosynthetic gene AtGA3ox1 (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1365-313X.2009.04055.x (literal)
- Alternative label
Gabriele, S.; Rizza, A.; Martone, J.; Circelli, P.; Costantino, P.; Vittorioso, P. (2010)
The Dof protein DAG1 mediates PIL5 activity on seed germination by negatively regulating the GA biosynthetic gene AtGA3ox1
in Plant journal (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Gabriele, S.; Rizza, A.; Martone, J.; Circelli, P.; Costantino, P.; Vittorioso, P. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dip Biologia e Biotecnologie, Sapienza Università di Roma (literal)
- Titolo
- The Dof protein DAG1 mediates PIL5 activity on seed germination by negatively regulating the GA biosynthetic gene AtGA3ox1 (literal)
- Abstract
- We have previously shown that inactivation of the gene encoding the Arabidopsis thaliana transcription factor
DOF AFFECTING GERMINATION 1 (DAG1) renders seed germination more sensitive to both phytochrome B
(phyB) and gibberellins (GA). dag1 mutant seeds require less red (R) light fluence and a lower GA concentration
than WT to germinate. Here, we show that inactivation of the gene PHYTOCHROME INTERACTING FACTOR
3-LIKE 5 (PIL5) results in down-regulation of DAG1. Inactivation of PIL5 in the dag1 mutant background further
increased the germination potential of dag1 mutant seeds, supporting the suggestion that DAG1 is under the
positive control of PIL5. Germination of dag1phyB seeds showed a reduced requirement of gibberellins as
compared with phyB mutant seeds, both in the presence and in the absence of GA biosynthesis. Furthermore,
the GA biosynthetic gene AtGA3ox1 is upregulated in dag1 seeds as compared with the WT, and DAG1 actually
binds to the AtGA3ox1 promoter, as shown by chromatin immunoprecipitation experiments. Expression
analysis at different time points confirms that AtGA3ox1 is directly regulated by DAG1, while suggesting that
DAG1 is not a direct regulatory target of PIL5. Our data indicate that in the phyB pathway leading to seed
germination, DAG1 negatively regulates GA biosynthesis and suggest that DAG1 acts downstream of PIL5. In
addition, the analysis of hypocotyls of dag1 and phyB mutant plantlets, of plantlets overexpressing phyB in
the dag1 mutant, as well as of dag1phyB double mutant suggests that DAG1 may act as a negative regulatory
element downstream of phyB also in hypocotyl elongation. (literal)
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