http://www.cnr.it/ontology/cnr/individuo/prodotto/ID185903
UVB radiation induced effects on cells studied by FTIR spectroscopy (Articolo in rivista)
- Type
- Label
- UVB radiation induced effects on cells studied by FTIR spectroscopy (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s00249-009-0446-9 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- L. Di Giambattista1; P. Grimaldi2; S. Gaudenzi3; D. Pozzi4; M. Grandi5; S. Morrone6; I. Silvestri7; A. Congiu Castellano8 (literal)
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- Rivista
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- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1,2,3,8=Dipartimento di Fisica, Università di Roma Sapienza, Piaz.le A. Moro, 2, 00185 Rome, Italy;
1=CISB (Interdepartmental Research Centre for Biomedical Systems Models and Information Analysis), Rome, Italy;
4,6,7=Dipartimento di Medicina Sperimentale, Università di Roma Sapienza, V.le Regina Elena, 324, 00161 Rome, Italy
5=Poliambulatorio La Torre, V. M. Ponzio,10, 10141 Torino, Italy (literal)
- Titolo
- UVB radiation induced effects on cells studied by FTIR spectroscopy (literal)
- Abstract
- Abstract We have made a preliminary analysis of the
results about the eVects on tumoral cell line (lymphoid T cell
line Jurkat) induced by UVB radiation (dose of 310 mJ/cm2)
with and without a vegetable mixture. In the present study,
we have used two techniques: Fourier transform infrared
spectroscopy (FTIR) and Xow cytometry. FTIR spectroscopy
has the potential to provide the identiWcation of the
vibrational modes of some of the major compounds (lipid,
proteins and nucleic acids) without being invasive in the
biomaterials. The second technique has allowed us to
perform measurements of cytotoxicity and to assess the
percentage of apoptosis. We already studied the induction
of apoptotic process in the same cell line by UVB radiation;
in particular, we looked for correspondences and correlations
between FTIR spetroscopy and Xow cytometry data
Wnding three highly probable spectroscopic markers of
apoptosis (Pozzi et al. in Radiat Res 168:698-705, 2007).
In the present work, the results have shown signiWcant
changes in the absorbance and spectral pattern in the wavenumber
protein and nucleic acids regions after the treatments. (literal)
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