http://www.cnr.it/ontology/cnr/individuo/prodotto/ID184745
A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance (Articolo in rivista)
- Type
- Label
- A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1038/ncb1774 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Yang J.S.; Gad H.; Lee S.Y.; Mironov A.; Zhang L.; Beznoussenko G.V.; Valente C.; Turacchio G.; Bonsra A.N.; Du G.; Baldanzi G.; Graziani A.; Bourgoin S.; Frohman M.A.; Luini A.; Hsu V.W. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Harvard Univ, Sch Med, Div Rheumatol Allergy & Immunol, Brigham & Womens Hosp, Boston, MA 02115 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA
Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Chieti, Italy
SUNY Stony Brook, Dept Pharmacol, Stony Brook, NY 11794 USA
SUNY Stony Brook, Ctr Dev Genet, Stony Brook, NY 11794 USA
Univ Piemonte Orientale, Dept Clin & Expt Med, I-28100 Novara, Italy
7. Ctr Hosp Univ Quebec, Pavillon CHUL, Quebec City, PQ G1V 4G2, Canada (literal)
- Titolo
- A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance (literal)
- Abstract
- Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation. (literal)
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