http://www.cnr.it/ontology/cnr/individuo/prodotto/ID184721
The closure of Pak1-dependent macropinosomes requires the phosphorylation of CtBP1/BARS (Articolo in rivista)
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- Label
- The closure of Pak1-dependent macropinosomes requires the phosphorylation of CtBP1/BARS (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1038/emboj.2008.59 (literal)
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- Liberali P.; Kakkonen E.; Turacchio G.; Valente C.; Spaar A.; Perinetti G.; Böckmann R.A.; Corda D.; Colanzi A.; Marjomaki V.; Luini A. (literal)
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- ISI Web of Science (WOS) (literal)
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- Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol, Lab Cell Regulat, I-66030 Santa Maria Imbaro, Italy
Univ Jyvaskyla, Nanosci Ctr, Dept Biol & Environm Sci, Jyvaskyla, Finland
Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, Lab Membrane Traff, I-66030 Santa Maria Imbaro, Italy
Univ Saarland, Ctr Bioinformat, D-6600 Saarbrucken, Germany (literal)
- Titolo
- The closure of Pak1-dependent macropinosomes requires the phosphorylation of CtBP1/BARS (literal)
- Abstract
- Membrane fission is an essential process in membrane trafficking and other cellular functions. While many fissioning and trafficking steps are mediated by the large GTPase dynamin, some fission events are dynamin independent and involve C-terminal-binding protein-1/brefeldinA-ADP ribosylated substrate (CtBP1/BARS). To gain an insight into the molecular mechanisms of CtBP1/BARS in fission, we have studied the role of this protein in macropinocytosis, a dynamin-independent endocytic pathway that can be synchronously activated by growth factors. Here, we show that upon activation of the epidermal growth factor receptor, CtBP1/BARS is (a) translocated to the macropinocytic cup and its surrounding membrane, (b) required for the fission of the macropinocytic cup and (c) phosphorylated on a specific serine that is a substrate for p21-activated kinase, with this phosphorylation being essential for the fission of the macropinocytic cup. Importantly, we also show that CtBP1/BARS is required for macropinocytic internalization and infection of echovirus 1. These results provide an insight into the molecular mechanisms of CtBP1/BARS activation in membrane fissioning, and extend the relevance of CtBP1/BARS-induced fission to human viral infection. (literal)
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