The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jnr.22528 (literal)

Alternative label

• Di Domenico F; Owen JB; Sultana R; Sowell RA ; Perluigi M; Cini C; Cai JA; Pierce WD; Butterfield DA (2010)
  The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression
  in Journal of neuroscience research; WILEY-LISS, DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Di Domenico F; Owen JB; Sultana R; Sowell RA ; Perluigi M; Cini C; Cai JA; Pierce WD; Butterfield DA (literal)

Pagina inizio

• 3566 (literal)

Pagina fine

• 3577 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 88 (literal)

Rivista

• Journal of neuroscience research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 16 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Kentucky, Ctr Membrane Sci, Dept Chem, Lexington, KY 40506 USA 2. Univ Kentucky, Sanders Brown Ctr Aging, Lexington, KY 40506 USA 3. Univ Roma La Sapienza, Dept Biochem Sci, I-00185 Rome, Italy 4. Univ Louisville, Dept Pharmacol, Louisville, KY 40292 USA (literal)

Titolo

• The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression (literal)

Abstract

• Lectin affinity chromatography is a powerful separation technique that fractionates proteins by selectively binding to specific carbohydrate moieties characteristic of protein glycosylation type. Wheat germ agglutinin (WGA) selectively binds terminal N-acetylglucosamine (O-GlcNAc) and sialic acid moieties characteristic of O-linked glycosylation. The current study utilizes WGA affinity chromatography to fractionate proteins from hippocampus and inferior parietal lobule (IPL) from subjects with Alzheimer's disease (AD) and arguably its earliest form, mild cognitive impairment (MCI). Proteins identified by proteomics that were fractionated from MCI and AD hippocampus by WGA affinity chromatography with altered levels compared with age-matched controls included GP96, gamma-enolase, glutamate dehydrogenase, glucosidase Il alpha, 14-3-3 epsilon, 14-3-3-gamma, 14-3-3 zeta, tropomyosin-2, calmodulin 2, gelsolin, beta-synuclein, alpha 1-antichymotrypsin, and dimethylguanosine tRNA methyltransferase. Proteins identified by proteomics that were fractionated from MCI and AD IPL by WGA affinity chromatography showing altered levels compared with age-matched controls included protein disulfide isomerase, calreticulin, and GP96. The proteins described in this study are involved in diverse processes, including glucose metabolism, endoplasmic reticulum (ER) functions, chaperoning, cytoskeletal assembly, and proteolysis, all of which are affected in AD. This study, the first to use proteomics to identify WGA-fractionated proteins isolated from brains from subjects with MCI and AD, provides additional information about the active proteome of the brain throughout AD progression. (literal)

Editore

• WILEY-LISS, DIV JOHN WILEY & SONS INC, 111 RIVER ST (Editore)

Prodotto di

• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• Chiara Cini (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Biologia strutturale e bioinformatica: struttura-funzione, dinamica e riconoscimento in proteine (SV.P14.008.001) (Modulo)

Autore CNR di

• Chiara Cini (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of neuroscience research (Rivista)

Editore di

• WILEY-LISS, DIV JOHN WILEY & SONS INC, 111 RIVER ST (Editore)

Insieme di parole chiave di

• Keywords of "The Wheat Germ Agglutinin-Fractionated Proteome of Subjects With Alzheimer's Disease and Mild Cognitive Impairment Hippocampus and Inferior Parietal Lobule: Implications for Disease Pathogenesis and Progression" (Insieme di parole chiave)