Good Caco-2 Cell Culture Practices (Articolo in rivista)

Type
Label
  • Good Caco-2 Cell Culture Practices (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.tiv.2012.03.009 (literal)
Alternative label
  • Natoli Manuela; Leoni Bruno D.; D'Agnano Igea; Zucco Flavia; Armando Felsani (2012)
    Good Caco-2 Cell Culture Practices
    in Toxicology in vitro
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Natoli Manuela; Leoni Bruno D.; D'Agnano Igea; Zucco Flavia; Armando Felsani (literal)
Pagina inizio
  • 1243 (literal)
Pagina fine
  • 1246 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Mar 23 [Epub ahead of print] (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 26 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 4 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 8 (literal)
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Institute of Cell Biology and Neurobiology, Via del Fosso di Fiorano 64, 00143 Rome, Italy (literal)
Titolo
  • Good Caco-2 Cell Culture Practices (literal)
Abstract
  • The human Caco-2 cells differentiate spontaneously in culture forming monolayers of mature intestinal 18 enterocytes which have been used as a model of the intestinal barrier for in vitro toxicology studies. 19 Reproducibility problems often reported in literature have been generally ascribed to different culture- 20 related conditions, such as the type of animal serum used, the supplements added to the culture media, 21 the passage number and the source of cell clones. 22 The Caco-2 cell culture protocol here described has been recently optimized in our laboratory, produc- 23 ing a homogeneous and highly polarized monolayer of cells which display many of the characteristics of 24 the intestinal enterocytes. This protocol differs from standard protocols mainly because Caco-2 cells are 25 subcultured when they reach just 50% of confluence, instead of 80%, retaining a high proliferation poten- 26 tial. When this cell population is seeded at high density on filter inserts differentiates almost synchro- 27 nously and much more homogenously. (literal)
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