http://www.cnr.it/ontology/cnr/individuo/prodotto/ID184160
Good Caco-2 Cell Culture Practices (Articolo in rivista)
- Type
- Label
- Good Caco-2 Cell Culture Practices (Articolo in rivista) (literal)
- Anno
- 2012-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.tiv.2012.03.009 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Natoli Manuela; Leoni Bruno D.; D'Agnano Igea; Zucco Flavia; Armando Felsani (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Mar 23 [Epub ahead of print] (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR, Institute of Cell Biology and Neurobiology, Via del Fosso di Fiorano 64, 00143 Rome, Italy (literal)
- Titolo
- Good Caco-2 Cell Culture Practices (literal)
- Abstract
- The human Caco-2 cells differentiate spontaneously in culture forming monolayers of mature intestinal 18
enterocytes which have been used as a model of the intestinal barrier for in vitro toxicology studies. 19
Reproducibility problems often reported in literature have been generally ascribed to different culture- 20
related conditions, such as the type of animal serum used, the supplements added to the culture media, 21
the passage number and the source of cell clones. 22
The Caco-2 cell culture protocol here described has been recently optimized in our laboratory, produc- 23
ing a homogeneous and highly polarized monolayer of cells which display many of the characteristics of 24
the intestinal enterocytes. This protocol differs from standard protocols mainly because Caco-2 cells are 25
subcultured when they reach just 50% of confluence, instead of 80%, retaining a high proliferation poten- 26
tial. When this cell population is seeded at high density on filter inserts differentiates almost synchro- 27
nously and much more homogenously. (literal)
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