Antimicrobial activity of immobilized lysozyme on plasma-treated polyethylene films (Articolo in rivista)

Type
Label
  • Antimicrobial activity of immobilized lysozyme on plasma-treated polyethylene films (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Conte, A; Buonocore, GG; Sinigaglia, M; Lopez, LC ; Favia, P ; D'Agostino, R ; Del Nobile, MA (2008)
    Antimicrobial activity of immobilized lysozyme on plasma-treated polyethylene films
    in Journal of food protection
    (literal)
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  • Conte, A; Buonocore, GG; Sinigaglia, M; Lopez, LC ; Favia, P ; D'Agostino, R ; Del Nobile, MA (literal)
Pagina inizio
  • 119 (literal)
Pagina fine
  • 125 (literal)
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  • 71 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ Foggia, Dept Food Sci, Foggia, Italy CNR, Inst Composite & Biomed Mat, I-80055 Naples, Italy Univ Foggia, Ist Ric & Appl Biotecnol Sicurezza Valorizzazz Pr, Foggia, Italy Univ Bari, Dept Chem, Bari, Italy (literal)
Titolo
  • Antimicrobial activity of immobilized lysozyme on plasma-treated polyethylene films (literal)
Abstract
  • In this study we tested the antimicrobial activity of polyethylene films modified by means of plasma processes that were followed by the chemical immobilization of lysozyme, an antimicrobial enzyme. To chemically immobilize the enzyme in its active form at the surface of polyethylene, substrates that had been plasma treated under different experimental conditions were soaked in lysozyme solutions at different concentrations. The immobilization of the enzyme was checked, and the antimicrobial activity of the films was investigated by observing the death rate of Micrococcus lysodeikticus cells suspended in phosphate buffer in contact with the films. The results clearly indicate that plasma-treated films loaded with lysozyme are active against the selected microorganism. A modified version of the Gompertz equation was used to quantitatively valuate the dependence of the antimicrobial activity of the films under both plasma treatment conditions and lysozyme concentrations. (literal)
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