http://www.cnr.it/ontology/cnr/individuo/prodotto/ID182977
Identification and characterization of the microvesicles released by muscle cells. (Contributo in atti di convegno)
- Type
- Label
- Identification and characterization of the microvesicles released by muscle cells. (Contributo in atti di convegno) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Alternative label
Paterniti G, Romancino D, Di Felice V, De Luca A, Campos YR, d'Azzo
A and Bongiovanni A. (2009)
Identification and characterization of the microvesicles released by muscle cells.
in VIIĀ° Congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo, Palermo, 17-18 Dicembre 2009
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Paterniti G, Romancino D, Di Felice V, De Luca A, Campos YR, d'Azzo
A and Bongiovanni A. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://www.unipa.it/dipbio/congresso2009/congresso2009lavori.html (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
- \"Excerpts from DBCS\"VIIĀ° Congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo 17-18 Dicembre 2009 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Paterniti G, Romancino D, Bongiovanni A.
Istituto di biomedicina e di immunologia molecolare \"A.Monroy\" (literal)
- Titolo
- Identification and characterization of the microvesicles released by muscle cells. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
- 978-88-904055-1-8 (literal)
- Abstract
- Several cell types have the capacity to secrete small
lipid vesicles that contain peculiar transmembrane
proteins, and enclose cytosolic components otherwise
excluded from the secretory pathway, because of their
lack of signal sequences. These vesicles function in
signalling and in the horizontal transfer of their
membrane proteins and/or cargo molecules, either
proteins or mRNAs. Two distinct mechanisms for the
release of two distinctive types of signalling vesicle
exist: the exocytosis of multivesicular bodies, with the
consequent release of the exosomes; and the direct
budding from the plasma membrane of small vesicles,
the shedding vesicles. The molecular composition
differs between the two types of these microvesicles,
and reflects the specialised functions of their cellular
origin. Here, we demonstrate that muscle cells secrete
microvesicles, which we characterized mainly as
exosomes. We describe the isolation and the partial
characterisation of exosomes released by proliferating
and differentiated striated muscle cells; their exosome
nature was confirmed by biochemical means and by
electron microscopy. Analysis of the vesicular protein
contents allowed us to identify the presence of proteins
such as: the E3 ubiquitin-ligase Ozz, the multifunctional
protein Alix/AIP1, the Enolase, and Chaperones
(hsp70). Alix is considered as an exosome marker, since
it is one of the most abundant exosome protein, as
demonstrated in B cells and dendritic cell-derived
exosomes (Mathivanan, S. and Simpson, 2009).
Muscle exosomes could represent a previously
undiscovered cellular communication system, which
could play an important role in the maintenance of
striated muscle homeostasis by synchronizing the
proliferation of myoblast, or the alignment and the
fusion of myotubes during muscle development and
regeneration.
Mathivanan, S. and Simpson, R.J. ExoCarta: A compendium of
exosomal proteins and RNA. Proteomics. 2009. 21, 4997-5000. (literal)
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