NGF regulates CGRP expression in human monocytes: effects on B7 expression and IL-10 production. (Abstract/Comunicazione in atti di convegno)

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  • NGF regulates CGRP expression in human monocytes: effects on B7 expression and IL-10 production. (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bracci-Laudiero L (1); Aloe L (1); Caroleo MC (2); Buanne P (3); Costa N (4); Starace G (1); Lundeberg T (5) (2004)
    NGF regulates CGRP expression in human monocytes: effects on B7 expression and IL-10 production.
    in 7th International Congress of the International-Society-of-Neuroimmunology, Venezia, Italia, 28 Settembre - 2 Ottobre 2004
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bracci-Laudiero L (1); Aloe L (1); Caroleo MC (2); Buanne P (3); Costa N (4); Starace G (1); Lundeberg T (5) (literal)
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  • 156 (literal)
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  • 154 (literal)
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  • 1 (literal)
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  • 1-2 (literal)
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  • Abstract (literal)
  • Comunicazione (literal)
  • ISI Web of Science (WOS) (literal)
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  • 1) Institute of Neurobiology and Molecular Medicine CNR, Rome, Italy; 2) the Department of Pharmacobiology, University of Calabria, Cosenza, Italy; 3) Deparment of Experimental Medicine, University of L'Aquila, L'Aquila, Italy; 4) Faculty of Pharmacy, University of Magna Grecia, Catanzaro, Italy; 5) Karolinska Institutet, Stockholm, Sweden. (literal)
Titolo
  • NGF regulates CGRP expression in human monocytes: effects on B7 expression and IL-10 production. (literal)
Abstract
  • Administration of Nerve Growth Factor (NGF) in EAE models delays the onset and prevents the full development of EAE lesions. In line with the newly found anti-inflammatory activity of NGF are our recent results on the autocrine NGF synthesis in B lymphocytes, which directly regulate in these cells the expression of calciotonin gene-related peptide (CGRP), a neuropeptide which is a potent inhibitor of T cell proliferation and antigen presentation by macrophages and monocytes. Using cultures of human monocytes we investigated by RT-PCR, immunofluorescence and flow cytometry analysis whether NGF can regulate the expression of CGRP also in these cells and influence the expression of B7.1 and B7.2 and IL-10. Our data indicate that monocytes synthesise basal levels of NGF but, after LPS stimulation, up-regulate NGF expression in dose-dependent fashion. When the cultures are deprived of endogenous NGF, CGRP expression in resting cells decreases and the up-regulation of CGRP induced by LPS is prevented. In addition we observed that endogenous NGF reduction affects co-stimulatory molecule expression and IL-10 synthesis, and these effects can be partially mimicked by using CGRP receptor I antagonist. Our findings indicate that endogenous synthesis of NGF in monocytes has a functional role and, by influencing B7.2 expression and IL-10 production, contributes to the down-regulation of the immune response and strongly support the newly found anti-inflammatory activity of NGF. (literal)
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