Automatic transwell assay by an EIS cell chip to monitor cell migration (Articolo in rivista)

Type
Label
  • Automatic transwell assay by an EIS cell chip to monitor cell migration (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/c1lc20540d (literal)
Alternative label
  • E. Primiceri; M. S. Chiriacò; F. Dioguardi; A. G. Monteduro; E. D'Amone; R. Rinaldi; G. Giannelli;G. Maruccio (2011)
    Automatic transwell assay by an EIS cell chip to monitor cell migration
    in Lab on a chip (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • E. Primiceri; M. S. Chiriacò; F. Dioguardi; A. G. Monteduro; E. D'Amone; R. Rinaldi; G. Giannelli;G. Maruccio (literal)
Pagina inizio
  • 4081 (literal)
Pagina fine
  • 4086 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://dx.doi.org/10.1039/c1lc20540d (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 11 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. NNL CNR Ist Nanosci, Lecce, Italy 2. Univ Salento, Scuola Super ISUFI, Lecce, Italy 3. Univ Salento, Dept Innovat Engn, Lecce, Italy 4. Univ Bari, Dept Internal Med Immunol & Infect Dis, Sect Internal Med, Sch Med, Bari, Italy 5. Univ Salento, Dept Phys, Lecce, Italy (literal)
Titolo
  • Automatic transwell assay by an EIS cell chip to monitor cell migration (literal)
Abstract
  • Here an EIS (electrochemical impedance spectroscopy) biochip to detect cell migration is demonstrated. This biochip has been inspired by a traditional transwell assay/modified Boyden chamber and consists of two compartments separated by a porous membrane. This structure (PDMS-based) is aligned to EIS sensors. Cells are seeded in the upper chamber through microfluidic channels. During migration cells go through the pores of the membrane and get in touch with the electrodes that detect migrated cells. The performance of our cell-chip was tested by investigating the migratory ability of hepatocellular carcinoma (HCC) cells as a function of microenvironment. For this purpose we challenged HCC cells to migrate on different extra-cellular matrix (ECM) components including laminin 1, collagen IV and laminin 5. The results reveal that our cell chip provides reliable results that consistently overlap with those obtained with traditional standardized Boyden chambers. Thus, we demonstrate a new, easy tool to study cell migration and to perform automatic assays. This approach is easier and faster than traditional transwell assays and can be suitable for high-throughput studies in drug discovery applications. (literal)
Prodotto di
Autore CNR

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it