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An Element of the Tertiary Structure of Peach Latent Mosaic Viroid RNA Revealed by UV Irradiation (Articolo in rivista)
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- An Element of the Tertiary Structure of Peach Latent Mosaic Viroid RNA Revealed by UV Irradiation (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1128/JVI.00630-06 (literal)
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Carmen Hernandez (1); Francesco Di Serio (2); Silvia Ambròs (3); José-Antonio Daròs (1) Ricardo Flores (1) (2006)
An Element of the Tertiary Structure of Peach Latent Mosaic Viroid RNA Revealed by UV Irradiation
in Journal of virology (Online); ASM, American society for microbiology, Washington, DC (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Carmen Hernandez (1); Francesco Di Serio (2); Silvia Ambròs (3); José-Antonio Daròs (1) Ricardo Flores (1) (literal)
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- (1) Instituto de Biologia Molecular y Celular de Plantas (UPV-CSIC), Universidad Politècnica de Valencia, Avenida de los Naranjos s/n,
46022 Valencia, Spain;
(2) Istituto di Virologia Vegetale del CNR, Sede di Bari, 70126 Bari, Italy
(3) Instituto Valenciano de Investigaciones Agrarias, Moncada 46113, Valencia, Spain (literal)
- Titolo
- An Element of the Tertiary Structure of Peach Latent Mosaic Viroid RNA Revealed by UV Irradiation (literal)
- Abstract
- Following UV irradiation, denaturing polyacrylamide gel electrophoresis and Northern blot hybridization
revealed a cross-link in Peach latent mosaic viroid (PLMVd) plus-strand RNA. Primer extension and partial
alkaline hydrolysis of the UV-irradiated PLMVd plus-strand RNA resulting from the hammerhead-mediated
self-cleavage mapped the cross-link at U81 and at the 3?-terminal C289 (or at a very proximal nucleotide).
Supporting this notion, in vitro-synthesized PLMVd plus-strand RNAs with short insertions/deletions at their
3? termini failed to cross-link. Because U81 and C289 are conserved in PLMVd variants and because the
initiation site of PLMVd minus-strand RNA maps at a short double-stranded motif containing C289, the
UV-photo-cross-linkable element of tertiary structure may be functionally significant. A second cross-linked
species similar in size and sequence to the monomeric circular PLMVd form, observed in some PLMVd
variants, probably derives from UV-induced ligation of the two termini resulting from self-cleavage. (literal)
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