http://www.cnr.it/ontology/cnr/individuo/prodotto/ID180832
Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac MRI in a rat model of myocardial infarction (Articolo in rivista)
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- Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac MRI in a rat model of myocardial infarction (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1152/ajpheart.00935.2010 (literal)
- Alternative label
Campan, Manuela [ 1 ]; Lionetti, Vincenzo [ 1,2 ]; Aquaro, Giovanni D. [ 2 ] ; Forini, Francesca [ 3 ]; Matteucci, Marco [ 1 ]; Vannucci, Laura [ 4,5 ] ; Chiuppesi, Flavia [ 4,5 ]; Di Cristofano, Claudio [ 8 ]; Faggioni, Michela [ 1 ] ; Maioli, Margherita [ 6,7 ] ; Barile, Lucio [ 9 ]; Messina, Elisa [ 10 ] ; Lombardi, Massimo; Pucci, Angela [ 11 ]; Pistello, Mauro [ 4,5 ]; Recchia, Fabio A. [ 1,12 ] (2011)
Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac MRI in a rat model of myocardial infarction
in American journal of physiology. Heart and circulatory physiology
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Campan, Manuela [ 1 ]; Lionetti, Vincenzo [ 1,2 ]; Aquaro, Giovanni D. [ 2 ] ; Forini, Francesca [ 3 ]; Matteucci, Marco [ 1 ]; Vannucci, Laura [ 4,5 ] ; Chiuppesi, Flavia [ 4,5 ]; Di Cristofano, Claudio [ 8 ]; Faggioni, Michela [ 1 ] ; Maioli, Margherita [ 6,7 ] ; Barile, Lucio [ 9 ]; Messina, Elisa [ 10 ] ; Lombardi, Massimo; Pucci, Angela [ 11 ]; Pistello, Mauro [ 4,5 ]; Recchia, Fabio A. [ 1,12 ] (literal)
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- ID_PUMA: cnr.ifc/2011-A0-140 (literal)
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- [ 1 ] CNR, Scuola Super Sant Anna, Sector Med, I-56127 Pisa, Italy; [ 2 ] CNR, Fondaz CNR Reg Toscana G Monasterio, I-56127 Pisa, Italy; [ 3 ] CNR, Ist Fisiol Clin, I-56127 Pisa, Italy; [ 4 ] Univ Pisa, Retrovirus Ctr, Pisa, Italy; [ 5 ] Univ Pisa, Dept Expt Pathol, Virol Sect, Pisa, Italy; [ 6 ] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy; [ 7 ] Univ Sassari, Natl Inst Biostruct & Biosyst, I-07100 Sassari, Italy, [ 8 ] Univ La Sapienza, Dept Expt Med, ICOT, Latina, Italy; [ 9 ] Univ Milano Bicocca, Dept Biotechnol & Biosci, Milan, Italy; [ 10 ] Univ Roma La Sapienza, Dept Expt Med, Rome, Italy; [ 11 ] Pisa Univ Hosp, Div Surg Mol & Ultrastruct Pathol, Pisa, Italy; [ 12 ] New York Med Coll, Dept Physiol, Valhalla, NY 10595 USA (literal)
- Titolo
- Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac MRI in a rat model of myocardial infarction (literal)
- Abstract
- The methods currently utilized to track stem cells by cardiac MRI are affected by important limitations, and new solutions are needed. We tested human ferritin heavy chain (hFTH) as a reporter gene for in vivo tracking of stem cells by cardiac MRI. Swine cardiac stem/progenitor cells were transduced with a lentiviral vector to overexpress hFTH and cultured to obtain cardiospheres (Cs). Myocardial infarction was induced in rats, and, after 45 min, the animals were subjected to intramyocardial injection of ~200 hFTH-Cs or nontransduced Cs or saline solution in the border zone. By employing clinical standard 1.5-Tesla MRI scanner and a multiecho T2* gradient echo sequence, we localized iron-accumulating tissue only in hearts treated with hFTH-Cs. This signal was detectable at 1 wk after infarction, and its size did not change significantly after 4 wk (6.33 ± 3.05 vs. 4.41 ± 4.38 mm 2). Cs transduction did not affect their cardioreparative potential, as indicated by the significantly better preserved left ventricular global and regional function and the 36% reduction in infarct size in both groups that received Cs compared with control infarcts. Prussian blue staining confirmed the presence of differentiated, iron-accumulating cells containing mitochondria of porcine origin. Cs-derived cells displayed CD31, ?-smooth muscle, and ?-sarcomeric actin antigens, indicating that the differentiation into endothelial, smooth muscle and cardiac muscle lineage was not affected by ferritin overexpression. In conclusion, hFTH can be used as a MRI reporter gene to track dividing/differentiating stem cells in the beating heart, while simultaneously monitoring cardiac morpho- functional changes. © 2011 the American Physiological Society. (literal)
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