http://www.cnr.it/ontology/cnr/individuo/prodotto/ID180817
Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (Articolo in rivista)
- Type
- Label
- Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1074/jbc.M111.268375 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- von Stockum S.; Basso E.; Petronilli V.; Sabatelli P.; Forte M.A.; Bernardi P. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- PubMe (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR Institute of Neuroscience and Department of Biomedical Sciences, University of Padova, Italy;
Institute of Molecular Genetics at the Istituto Ortopedico Rizzoli, Bologna, Italy;
Vollum Institute, Oregon Health and Sciences University, Portland, Oregon, USA (literal)
- Titolo
- Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (literal)
- Abstract
- We have studied the pathways for Ca2+ transport in mitochondria of the fruit fly Drosophila rnelanogaster. We demonstrate the presence of ruthenium red (RR)-sensitive Ca2+ uptake, of RR-insensitive Ca2+ release, and of Na+-stimulated Ca2+ release in energized mitochondria, which match well characterized Ca2+ transport pathways of mammalian mitochondria. Following larger matrix Ca2+ loading Drosophila mitochondria underwent spontaneous RR-insensitive Ca2+ release, an event that in mammals is due to opening of the permeability transition pore (PTP). Like the PIP of mammals, Drosophila Ca2+-induced Ca2+ release could be triggered by uncoupler, diamide, and N-ethylmaleimide, indicating the existence of regulatory voltage- and redox-sensitive sites and was inhibited by tetracaine. Unlike PTP-mediated Ca2+ release in mammals, however, it was (i) insensitive to cyclosporin A, ubiquinone 0, and ADP; (ii) inhibited by P-i, as is the PTP of yeast mitochondria; and (iii) not accompanied by matrix swelling and cytochrome c release even in KCl-based medium. We conclude that Drosophila mitochondria possess a selective Ca2+ release channel with features intermediate between the PTP of yeast and mammals. (literal)
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