Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (Articolo in rivista)

Type
Label
  • Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M111.268375 (literal)
Alternative label
  • von Stockum S.; Basso E.; Petronilli V.; Sabatelli P.; Forte M.A.; Bernardi P. (2011)
    Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster
    in The Journal of biological chemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • von Stockum S.; Basso E.; Petronilli V.; Sabatelli P.; Forte M.A.; Bernardi P. (literal)
Pagina inizio
  • 41163 (literal)
Pagina fine
  • 41170 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 286 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR Institute of Neuroscience and Department of Biomedical Sciences, University of Padova, Italy; Institute of Molecular Genetics at the Istituto Ortopedico Rizzoli, Bologna, Italy; Vollum Institute, Oregon Health and Sciences University, Portland, Oregon, USA (literal)
Titolo
  • Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster (literal)
Abstract
  • We have studied the pathways for Ca2+ transport in mitochondria of the fruit fly Drosophila rnelanogaster. We demonstrate the presence of ruthenium red (RR)-sensitive Ca2+ uptake, of RR-insensitive Ca2+ release, and of Na+-stimulated Ca2+ release in energized mitochondria, which match well characterized Ca2+ transport pathways of mammalian mitochondria. Following larger matrix Ca2+ loading Drosophila mitochondria underwent spontaneous RR-insensitive Ca2+ release, an event that in mammals is due to opening of the permeability transition pore (PTP). Like the PIP of mammals, Drosophila Ca2+-induced Ca2+ release could be triggered by uncoupler, diamide, and N-ethylmaleimide, indicating the existence of regulatory voltage- and redox-sensitive sites and was inhibited by tetracaine. Unlike PTP-mediated Ca2+ release in mammals, however, it was (i) insensitive to cyclosporin A, ubiquinone 0, and ADP; (ii) inhibited by P-i, as is the PTP of yeast mitochondria; and (iii) not accompanied by matrix swelling and cytochrome c release even in KCl-based medium. We conclude that Drosophila mitochondria possess a selective Ca2+ release channel with features intermediate between the PTP of yeast and mammals. (literal)
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