http://www.cnr.it/ontology/cnr/individuo/prodotto/ID180635

Inter-laboratory validation of PCR-based protocol for detection of olive viruses (Articolo in rivista)

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Inter-laboratory validation of PCR-based protocol for detection of olive viruses (Articolo in rivista) (literal)

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G. Loconsole; M. Saponari; F. Faggioli; G. Albanese; H. Bouyahia; T. Elbeaino; A. Materazzi; M. Nuzzaci; V. Prota; G. Romanazzi; N. Trisciuzzi; V. Savino (2010)
Inter-laboratory validation of PCR-based protocol for detection of olive viruses
in Bulletin OEPP (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

G. Loconsole; M. Saponari; F. Faggioli; G. Albanese; H. Bouyahia; T. Elbeaino; A. Materazzi; M. Nuzzaci; V. Prota; G. Romanazzi; N. Trisciuzzi; V. Savino (literal)

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Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://onlinelibrary.wiley.com/doi/10.1111/epp.2010.40.issue-3/issuetoc (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

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Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Dipartimento di Protezione delle Piante e Microbiologia Applicata - Universita` degli Studi di Bari, Via Amendola, 165 ? A, 70126 Bari (Italy); e-mail: giuliana.loconsole@agr.uniba.it CRA-Centro di Ricerca per la Patologia Vegetale, Via C.G. Bertero, 22, 00156 Roma (Italy) Dipartimento di Gestione dei Sistemi Agrari e Forestali, Universita` degli Studi Mediterranea di Reggio Calabria, Llocalita` Feo di Vito, 89060 Reggio Calabria (Italy) Dipartimento di Coltivazione e Difesa delle specie Legnose ''G. Scaramuzzi'', Universita` di Pisa, Via del Borghetto, 80, 56124 Pisa (Italy) Istituto Agronomico Mediterraneo, Via Ceglie, 9, 70010 Valenzano (Bari) (Italy) Dipartimento di Biologia, Difesa e Biotecnologie Agroforestali, Universita` degli Studi della Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza (Italy) Dipartimento di Protezione delle Piante, Sez. di Patologia Vegetale, Universita` degli Studi di Sassari, Via De Nicola, 1, 07100 Sassari (Italy) Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Universita` Politecnica delle Marche, via Brecce Bianche, 1, 60131 Ancona (Italy) Centro di Ricerca e Sperimentazione in Agricoltura 'Basile Caramia', Via Cisternino, 281, 70010 Locorotondo (Bari) (Italy) (literal)

Titolo

Inter-laboratory validation of PCR-based protocol for detection of olive viruses (literal)

Abstract

Sanitary selection and certification of olive cultivars require sensitive diagnostic methods and effective sanitation protocols. Although much attention has been paid in the past few years to the development of diagnostic tools for reliable virus identification, the need to define a common and standardized diagnostic protocol led to the implementation of a ring test among nine Italian diagnostic laboratories. A one-step RT-PCR protocol and different primer sets, targeting the most common olive viruses covered by phytosanitary rules, were tested in each laboratory, using the same batch of positive and healthy controls as well as the same amplification conditions and reaction components. The one-step RT-PCR, performed using several specific primer sets, was able efficiently to detect the target viruses in all laboratories. Furthermore, a one-step RT-PCR protocol was used successfully for the first time for detection of Tobacco necrosis virus (TNV) and Olive mild mosaic virus (OMMV). Results showed that all target viruses were not uniformly distributed in the canopy, and that at least two subsets of samples must be collected from each plant. This standardized protocol is now being used to produce nuclear stocks for 70 different Italian olive cultivars, in the framework of the national project OLVIVA, which involves 25 national research institutions. (literal)

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