Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1094/PHYTO-100-4-0319 (literal)

Alternative label

• R. K. Yokomi; M. Saponari; P. J. Sieburth (2010)
  Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays
  in Phytopathology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• R. K. Yokomi; M. Saponari; P. J. Sieburth (literal)

Pagina inizio

• 319 (literal)

Pagina fine

• 327 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 100 (literal)

Rivista

• Phytopathology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Parlier, CA 93648; Bureau of Citrus Budwood Registration, Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Winter Haven, FL 33881. (literal)

Titolo

• Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays (literal)

Abstract

• A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strainspecific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reaction. Probe CPi-VT3 was designed for VT and T3 genotypes; probe CPi-T36 for T36 genotypes; and probe CPi-T36-NS to identify isolates in an outgroup clade of T36-like genotypes mild in California. Total nucleic acids extracted by chromatography on silica particles, sodium dodecyl sulfatepotassium acetate, and CTV virion immunocapture all yielded high quality templates for real-time PCR detection of CTV. These assays successfully differentiated CTV isolates from California, Florida, and a large panel of CTV isolates from an international collection maintained in Beltsville, MD. The utility of the assay was validated using field isolates collected in California and Florida. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• MARIA SAPONARI (Persona)

Insieme di parole chiave

• Parole chiave di "Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARIA SAPONARI (Persona)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Phytopathology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Rapid Differentiation and Identification of Potential Severe Strains of Citrus tristeza virus by Real-Time Reverse Transcription-Polymerase Chain Reaction Assays" (Insieme di parole chiave)