http://www.cnr.it/ontology/cnr/individuo/prodotto/ID180408
Development of real-time PCR based assays for simultaneous and improved detection of citrus viruses (Articolo in rivista)
- Type
- Label
- Development of real-time PCR based assays for simultaneous and improved detection of citrus viruses (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s10658-010-9653-6 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Giuliana Loconsole; Maria Saponari; Vito Savino (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Protezione delle Piante e Microbiologia
Applicata, Università degli Studi Aldo Moro di Bari,
Bari, Italy (literal)
- Titolo
- Development of real-time PCR based assays for simultaneous and improved detection of citrus viruses (literal)
- Abstract
- Citrus, one of the most economically
important crops, is susceptible to a number of
arthropod- and graft-transmissible pathogens. Rapid
and reliable methods for detecting multiple pathogens
are important for routine diagnosis by reducing time,
labour and costs. To this end, primers and TaqMan
probes for Citrus psorosis virus (CPsV) and Citrus
variegation virus (CVV) detection by singleplex realtime
(q) reverse transcription (RT)- PCR were initially
designed. Further optimizations included the development
of a multiplex (m) RT-qPCR assay to detect
simultaneously CPsV, CVV, and Citrus tristeza virus
(CTV) in a single reaction. When 10-fold serial
dilutions prepared using total RNAs from CPsV- and
CVV-infected plants were tested, RT-qPCR assays
proved to be 100 and 1000 times more sensitive than
conventional RT-PCR, respectively. The target viruses
were effectively identified by mRT-qPCR in fieldinfected
clementine and sweet orange trees. The
optimized multiplex assay proved to be as sensitive
as the singleplex tests, thus providing a valuable
alternative tool for detection of these citrus viruses. (literal)
- Prodotto di
- Autore CNR
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