http://www.cnr.it/ontology/cnr/individuo/prodotto/ID179353

Mitochondria are primary targets in apoptosis induced by the mixed phosphine gold species chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) in melanoma cell lines. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Mitochondria are primary targets in apoptosis induced by the mixed phosphine gold species chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) in melanoma cell lines. (Articolo in rivista) (literal)

Anno

2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.bcp.2006.11.018 (literal)

Alternative label

CARUSO Francesco; VILLA Raffaella; ROSSI Miriam; PETTINARI Claudio; PADUANO Francesco; PENNATI Marzia; DAIDONE Maria Grazia; ZAFFARONI, Nadia (2007)
Mitochondria are primary targets in apoptosis induced by the mixed phosphine gold species chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) in melanoma cell lines.
in Biochemical pharmacology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

CARUSO Francesco; VILLA Raffaella; ROSSI Miriam; PETTINARI Claudio; PADUANO Francesco; PENNATI Marzia; DAIDONE Maria Grazia; ZAFFARONI, Nadia (literal)

Pagina inizio

773 (literal)

Pagina fine

781 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://dx.doi.org/10.1016/j.bcp.2006.11.018 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

73 (literal)

Rivista

Biochemical pharmacology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

6 (literal)

Note

ISI Web of Science (WOS) (literal)
Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Piazzale Aldo Moro 5, 00185 Rome, Italy; Dipartimento di Oncologia Sperimentale e Laboratori, Fondazione IRCCS Istituto Nazionale dei Tumori, Via Venezian 1, 20133 Milan, Italy; Vassar College, Department of Chemistry, Poughkeepsie, NY 12604-0484, USA; Dipartimento di Scienze Chimiche, Universita di Camerino, via S. Agostino 1, 62032 Camerino, MC, Italy (literal)

Titolo

Mitochondria are primary targets in apoptosis induced by the mixed phosphine gold species chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) in melanoma cell lines. (literal)

Abstract

Based on previous evidence indicating a selective cytotoxic activity of the mixed phosphine gold complex chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) for melanoma cells, we investigated the cellular bases of its antiproliferative effect in a panel of human melanoma cell lines (JR8, SK-Mel-5, Mel-501, 2/60, 2/21 and GRIG). The drug consistently induced a dose-dependent inhibition of cell growth, with IC50 values ranging from 0.8 to 2.3 mM and, when tested under the same experimental conditions, its cytotoxic activity was higher than (from 2- to 5-fold) or comparable to that of cisplatin as a function of cell lines. The ability of the gold complex to activate programmed cell death was assessed in JR8 and 2/60 cells, and a dose-dependent increase in cells with an apoptotic nuclear morphology was observed in both cell lines (up to 40 and 66% of the overall cell population, for JR8 and 2/60 cell lines, respectively). Such an apoptotic response was mediated by a dosedependent loss of mitochondrial membrane potential, cytochrome c and Smac/DIABLO release from mitochondria into cytosol and enhanced caspase-9 and caspase-3 catalytic activity. A reduced or completely abrogated expression of the anti-apoptotic proteins c-IAP1, XIAP and survivin in drug-treated cells was also observed. Overall, results from the study indicate that chlorotriphenylphosphine-1,3-bis(diphenylphosphino)propanegold(I) markedly inhibits melanoma cell growth by inducing mitochondria-mediated apoptosis and suggest it as a good candidate for additional evaluation as an anticancer agent against melanoma. (literal)

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