Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays (Articolo in rivista)

Type
Label
  • Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ab.2011.07.004 (literal)
Alternative label
  • Cretich, Marina; Bagnati, Marta; Damin, Francesco; Sola, Laura; Chiari, Marcella (2011)
    Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays
    in Analytical biochemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cretich, Marina; Bagnati, Marta; Damin, Francesco; Sola, Laura; Chiari, Marcella (literal)
Pagina inizio
  • 164 (literal)
Pagina fine
  • 166 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 418 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 3 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WoS) (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Consiglio Nazionale delle Ricerche (CNR) (literal)
Titolo
  • Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays (literal)
Abstract
  • Here we combine the use of fluorescence-enhancing silicon substrates coated by copoly(DMA-NAS-MAPS), a ter-copolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS), with an efficient dynamic incubation to overcome mass transport limitations and obtain femtomolar limits of detection. The high sensitivity was obtained with a conventional microarray scanner without the use of any sophisticated detection strategy or protocol. When the method was applied, an improvement of the analytical sensitivity of approximately three orders of magnitude was achieved for antibody detection when compared with the same assay performed on regular glass slides and static conditions. Moreover, limits of detection of 45 and 54 pg/ml were obtained for hepatitis B superficial antigen and HIV p24 antigen, respectively. (C) 2011 Elsevier Inc. All rights reserved. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it