Bright oligothiophene N-succinimidyl esters for efficient labeling of proteins and oligonucleotides (Articolo in rivista)

Type
Label
  • Bright oligothiophene N-succinimidyl esters for efficient labeling of proteins and oligonucleotides (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/bc050250a (literal)
Alternative label
  • G. Barbarella ; M. Zambianchi ; A. Ventola ; E. Fabiano ; F. Della Sala ; G. Gigli ; M. Anni ; A. Bolognesi ; L. Polito ; M. Naldi ; M. Capobianco (2006)
    Bright oligothiophene N-succinimidyl esters for efficient labeling of proteins and oligonucleotides
    in Bioconjugate chemistry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • G. Barbarella ; M. Zambianchi ; A. Ventola ; E. Fabiano ; F. Della Sala ; G. Gigli ; M. Anni ; A. Bolognesi ; L. Polito ; M. Naldi ; M. Capobianco (literal)
Pagina inizio
  • 58 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://pubs.acs.org/doi/abs/10.1021/bc050250a (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 17 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Consiglio Nazionale Ricerche (ISOF) Mediteknology srl, Area Ricerca CNR, Via Gobetti 101, I-40129 Bologna, Italy National Nanotechnology Laboratory (NNL), Distretto Tecnologico, Via Arnesano, I-73100 Lecce, Italy, Dipartimento di Patologia Sperimentale, Università di Bologna, Via San Giacomo, 14, I-40126 Bologna, Italy (literal)
Titolo
  • Bright oligothiophene N-succinimidyl esters for efficient labeling of proteins and oligonucleotides (literal)
Abstract
  • The synthesis of multicolor fluorescent oligothiophene N-succinimidyl esters (TSEs) is reported, and their optical properties are discussed with the aid of ab initio calculations. The esters were coupled to proteins and to 3'-amino-modified oligonucleotides in mild conditions and with similar modalities. A comparative study of the bioconjugate of IgG1 anti-CD3 antibody labeled with a blue fluorescent TSE and with fluorescein isothiocyanate (FITC) is reported, showing that the former achieves higher photoluminescence intensity and optical stability than the latter. Fluorescence resonance energy transfer experiments with TSE-labeled oligonucleotides and examples of cellular imaging via TSE-labeled proteins are reported. (literal)
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