http://www.cnr.it/ontology/cnr/individuo/prodotto/ID177433

Chemical-physical characterization of polyhydroxyalkanoates recovered by means of a simplified method from cultures of Halomonas campaniensis. (Articolo in rivista)

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Chemical-physical characterization of polyhydroxyalkanoates recovered by means of a simplified method from cultures of Halomonas campaniensis. (Articolo in rivista) (literal)

Anno

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Alternative label

G. Strazzullo; A. Gambacorta; F. M. Vella; B. Immirzi; I. Romano; V. Calandrelli; B. Nicolaus; L. Lama (2008)
Chemical-physical characterization of polyhydroxyalkanoates recovered by means of a simplified method from cultures of Halomonas campaniensis.
in World journal of microbiology & biotechnology; SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

G. Strazzullo; A. Gambacorta; F. M. Vella; B. Immirzi; I. Romano; V. Calandrelli; B. Nicolaus; L. Lama (literal)

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Istituto di Scienze delle Preparazioni Alimentari (ISPA) CNR, Via L. Da Vinci 44, 10095 Grugliasco, To, Italy; Istituto di Chimica Biomolecolare (ICB) CNR, Via Campi Flegrei 34, 80078 Pozzuoli, Na, Italy; Istituto di Chimica e Tecnologia dei Polimeri (ICTP) CNR, Via Campi Flegrei 34, 80078 Pozzuoli, Na, Italy. (literal)

Titolo

Chemical-physical characterization of polyhydroxyalkanoates recovered by means of a simplified method from cultures of Halomonas campaniensis. (literal)

Abstract

In this study we suggest a simplified and effective method to directly recover polyhydroxyalkanoates (PHAs) from humid biomass of Halomonas campaniensis with no pre-treatment steps. Sodium dodecyl sulphate (SDS) was directly added to dispersed biomass of cultured micro-organism (w/w ratio: 1) in distilled water followed by shaking, heat treatment, and washing steps. The purity of the recovered PHAs synthesized by H. campaniensis was over 95%, regardless of the cell concentrations and the best yield was 12% (w/w) of the cell wet weight when the micro-organism was cultivated in a glucose-based medium or a glucose/propionate-based medium. MS spectroscopy and 1H, 13C-NMR analysis were used to chemically characterize the PHAs; their thermal characteristics were obtained using a differential scanning calorimeter and the average viscosity molecular weight was assessed through specific viscosity measurements. Due to its ease and velocity, our simplified method is suitable for the detection and recovery of PHAs from humid biomasses with high yield and purity. The method, which is quick and at low environmental impact, is very valuable for the simultaneous testing of cultures grown with different inducers for PHAs having particular chemical/physical characteristics. (literal)

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