Adaption versus shock response to PEG-induced low water potential in cultured potato cell (Articolo in rivista)

Type
Label
  • Adaption versus shock response to PEG-induced low water potential in cultured potato cell (Articolo in rivista) (literal)
Anno
  • 1994-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1034/j.1399-3054.1994.920104.x (literal)
Alternative label
  • Leone A.; Costa A.; Tucci M;, Grillo S. (1994)
    Adaption versus shock response to PEG-induced low water potential in cultured potato cell
    in Physiologia plantarum (Kbh., 1948)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Leone A.; Costa A.; Tucci M;, Grillo S. (literal)
Pagina inizio
  • 21 (literal)
Pagina fine
  • 30 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 92 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Genetica Vegetale (literal)
Titolo
  • Adaption versus shock response to PEG-induced low water potential in cultured potato cell (literal)
Abstract
  • We compared long-term adaptation versus short-term or shock response of potato (Solanum tuberosum) cells to polyethylene glycol (PEG)-induced low water potential. Potato cells, which were allowed to adapt gradually to a decreasing water potential, were able to grow actively in a medium containing 20% PEG. In contrast, no appreciable gain in dry weight was observed in potato cells shocked by abrupt transfer to the same medium. PEG-adapted cells were also sail-tolerant, as they were able to proliferate in a medium supplemented with 200 mM NaCl. No visible ultrastructural changes of mitochondria or proplastids were observed in adapted cells at values of low water potential (about -2.0 MPa), which caused membrane disruption and appearance of lipid droplets in unadapted cells. ABA cellular content increased 5-fold in PEG-shocked cells but no significant increase was found in PEG-adapted cells. The intracellular content of free proline increased 12.5 times over the basal level in PEG-adapted cells and 6.5 times in PEG-shocked cells. As shown by in vivo protein labeling, shock conditions strongly inhibited protein synthesis, which was completely recovered in PEG-adapted cells. Osmotin, a protein associated with salt adaptation in tobacco, was constitutively expressed at a high level in PEG-adapted cells and accumulated in PEG-shocked cells only three days after the transfer in a medium supplemented with 20% PEG. Proline and osmotin accumulation were coincident with the increase in cellular ABA content in PEG-shocked cells, but not in PEG-adapted cells. These data suggest that this hormone is mainly involved in shock response rather than long-term adaptation. (literal)
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