Coating of nitrocellulose for colorimetric DNA microarrays (Articolo in rivista)

Type
Label
  • Coating of nitrocellulose for colorimetric DNA microarrays (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cretich M.; Sedini V.; Damin F.; Pelliccia M.; Sola L.; Chiari M. (2010)
    Coating of nitrocellulose for colorimetric DNA microarrays
    in Analytical biochemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cretich M.; Sedini V.; Damin F.; Pelliccia M.; Sola L.; Chiari M. (literal)
Pagina inizio
  • 84 (literal)
Pagina fine
  • 88 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 397 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Chimica del Riconoscimento Molecolare (ICRM), CNR (literal)
Titolo
  • Coating of nitrocellulose for colorimetric DNA microarrays (literal)
Abstract
  • We report on the modification of a nitrocellulose film with copoly(DMA-NAS-MAPS), a tercopolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS). The chains of this polymer, interacting with nitrocellulose fibers, introduce active ester functionalities that Promote the covalent binding of short oligonucleotide fragments to the nitrocellulose thin film. Using colorimetric detection, naked eye visible DNA microarrays are developed for easy identification of foodborne pathogens. The fast and robust procedure of nitrocellulose functionalization opens the opportunity to implement this material in disposable analytical microdevices that do not require sophisticated readout systems. (literal)
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