APEX protocol implementation on a lab-on-a-chip for SNPs detection (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• APEX protocol implementation on a lab-on-a-chip for SNPs detection (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.mee.2007.12.024 (literal)

Alternative label

• Marasso S.L. a; Canavese G. a; Cocuzza M. a; Ferrarini A. c; Giuri E. a; Lo Bartolo S. b; Mantero G. b; Perrone D. a; Quaglio M. a; Vallini I. b (2008)
  APEX protocol implementation on a lab-on-a-chip for SNPs detection
  in Microelectronic engineering; North Holland Pub. Co., Amsterdam (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Marasso S.L. a; Canavese G. a; Cocuzza M. a; Ferrarini A. c; Giuri E. a; Lo Bartolo S. b; Mantero G. b; Perrone D. a; Quaglio M. a; Vallini I. b (literal)

Pagina inizio

• 1326 (literal)

Pagina fine

• 1329 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 85 (literal)

Rivista

• Microelectronic engineering (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5-6 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a Chilab - Materials and Microsystems Laboratory, Polytechnic of Turin - Latemar Unit, Chivasso (Turin) 10034, Italy; b Biodiversity S.p.A. - Latemar Unit, Brescia 25124, Italy; c Dipartimento Scientifico e Tecnologico, Universita` di Verona - Latemar Unit, Verona 37134, Italy (literal)

Titolo

• APEX protocol implementation on a lab-on-a-chip for SNPs detection (literal)

Abstract

• In this work a detection module for the single nucleotide polymorphisms (SNPs) detection was realised. In particular arrayed primer extension (APEX) was selected as innovative method for SNPs detection and this protocol was scaled down following a micro total analysis approach in order to fabricate a lab-on-a-chip (LOC). Finite element analysis and behavioural simulations with commercial tools to properly design the microfluidic circuitry have preceded the technological processes for the production of the device. The fluidic was designed to contain 5 ll of DNA and reagents that are inserted through three inlets. The layout includes two mixers and a sealed reaction chamber. Glass/silicon prototypes were fabricated with the employment of micro-electro-mechanical-system (MEMS) processes. The devices were tested with APEX biological protocols customized for the scaled volumes. Furthermore, in order to demonstrate the absence of any negative interaction between the chip and the APEX reagents, different chips were tested in intermediate steps together with the protocol executed in standard conditions. The final result demonstrates that the thermo sequenase extended the probes with the dideoxynucleotides modified with Cy5 fluorophore, thus indicating the possibility of implementation of the APEX protocol on LOC devices. (literal)

Editore

• North Holland Pub. Co. (Editore)

Prodotto di

• Istituto dei materiali per l'elettronica ed il magnetismo (IMEM) (Istituto)

Autore CNR

• MATTEO COCUZZA (Unità di personale interno)

Insieme di parole chiave

• Keywords of "APEX protocol implementation on a lab-on-a-chip for SNPs detection" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto dei materiali per l'elettronica ed il magnetismo (IMEM) (Istituto)

Autore CNR di

• MATTEO COCUZZA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Microelectronic engineering (Rivista)

Editore di

• North Holland Pub. Co. (Editore)

Insieme di parole chiave di

• Keywords of "APEX protocol implementation on a lab-on-a-chip for SNPs detection" (Insieme di parole chiave)