http://www.cnr.it/ontology/cnr/individuo/prodotto/ID17693
Isolation and characterization of a myotoxin from the venom of Macrovipera Lebetina transmediterranea (Articolo in rivista)
- Type
- Label
- Isolation and characterization of a myotoxin from the venom of Macrovipera Lebetina transmediterranea (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.toxicon.2010.04.001 (literal)
- Alternative label
Hamza L., Girardi T., Castelli S., Gargioli C., Cannata S., Patamia M., Luly P., Laraba-Djebari F., Petruzzelli R., Rufini S. (2010)
Isolation and characterization of a myotoxin from the venom of Macrovipera Lebetina transmediterranea
in Toxicon (Oxf.)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Hamza L., Girardi T., Castelli S., Gargioli C., Cannata S., Patamia M., Luly P., Laraba-Djebari F., Petruzzelli R., Rufini S. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Loubna Hamza a, Tiziana Girardi b, Silvia Castelli c, Cesare Gargioli d, Stefano Cannata c,
Maria Patamia e, Paolo Luly c, Fatima Laraba-Djebari a, Raffaele Petruzzelli f, Stefano Rufini c,
a Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie \"Houari Boumédienne\" Bab Ezzouar, Alger, Algeria
b Department of Molecular and Developmental Genetics, Flanders Institute for Biotechnology (VIB), B-3000 Leuven, Belgium
c Department of Biology, University of Rome \"Tor Vergata\", Via della Ricerca Scientifica, snc, 000133 Roma, Italy
d San Raffaele Biomedical Park Foundation, Via di Castel Romano 100, Rome, Italy
e Institute of Chemistry of Molecular Recognition, National Research Council (CNR), Rome, Italy
f Department of Biomedical Sciences, University \"G. D'Annunzio\" Chieti, Italy (literal)
- Titolo
- Isolation and characterization of a myotoxin from the venom of Macrovipera Lebetina transmediterranea (literal)
- Abstract
- The Macrovipera lebetina venom consists of a complex mixture of proteins belonging to
a few main families according to their enzymatic and pharmacological activity. Given the
serious pathophysiological effects caused by M. lebetina bites mainly induced by muscle
degeneration, we decided to investigate the myotoxic activity of some venom fractions. In
the present study we describe the purification and characterization of a 22.600 kDa
protein, named in the following Mlp4.2, that shares myotoxic but not haemorrhagic
activity in vivo. Herein we report that Mlp4.2 is a metalloproteinase belonging to the PISVMPS
family able, in vitro, to proteolyse extracellular matrix proteins as laminin and
fibronectin. Histological observations of mouse anterior tibialis Mlp4.2-treated muscle,
demonstrate that this protein induces a massive degeneration of myofibers but not haemorrhage.
The immunofluorescence analysis of protein-treated anterior tibialis, demonstrates
that Mlp4.2 is able to disarray the laminin network surrounding muscle fibers.
Finally Mlp4.2 did not show any direct cytolytic activity towards the myogenic cell line
C2C12 in culture. The data reported herein suggest that the myotoxicity of Mlp4.2 is
primarily linked to the disruption of the muscle fibers interaction with extracellular matrix
proteins. (literal)
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