Antifungal-protein production in maize (Zea mays) suspension cultures (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Antifungal-protein production in maize (Zea mays) suspension cultures (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• Perri F., Della Penna S., Rufini F., Patamia M., Bonito M., Angiolella L., Vitali A. (2009)
  Antifungal-protein production in maize (Zea mays) suspension cultures
  in Biotechnology and applied biochemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Perri F., Della Penna S., Rufini F., Patamia M., Bonito M., Angiolella L., Vitali A. (literal)

Pagina inizio

• 273 (literal)

Pagina fine

• 281 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 52 (literal)

Rivista

• Biotechnology and applied biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Chimica del Riconoscimento Molecolare, uos Roma, c/o Istituto di Biochimica e Biochimica Clinica, Università Cattolica, L,go F. Vito, 1 00168, Roma (literal)

Titolo

• Antifungal-protein production in maize (Zea mays) suspension cultures (literal)

Abstract

• The growing emergency due to the phenomenon of drug resistance to micro-organisms has pushed forward the search for new potential drug alternatives to those already in use. Plants represent a suitable source of new antifungal molecules, as they produce a series of defensive proteins. Among them are the PRPs (pathogenesis-related proteins), shown to be effective in vitro against human pathogens. An optimized and established cell-suspension culture of maize (Zea mays) was shown to constitutively secrete in the medium a series of PRPs comprising the antifungal protein zeamatin (P33679) with a final yield of approx. 3 mg/litre. The in-vitro-produced zeamatin possessed antifungal activity towards a clinical strain of the human pathogenic yeast Candida albicans, an activity comparable with the one reported for the same protein extracted from maize seeds. Along with zeamatin, other PRPs were expressed: a 9 kDa lipid-transfer protein, a 26 kDa xylanase inhibitor and a new antifungal protein, PR-5. A fast, two-step chromatographic procedure was set up allowing the complete purification of the proteins considered, making this cell line a valuable system for the production of potential antifungal agents in a reliable and easy way. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)

Autore CNR

• ALBERTO VITALI (Unità di personale interno)
• MARIA PATAMIA (Persona)

Incoming links:

Autore CNR di

• ALBERTO VITALI (Unità di personale interno)
• MARIA PATAMIA (Persona)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biotechnology and applied biochemistry (Rivista)