Mitochondrial oxygen consumption inhibition importance for TMT-dependent cell death in undifferentiated PC12 cells (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Mitochondrial oxygen consumption inhibition importance for TMT-dependent cell death in undifferentiated PC12 cells (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Misiti F., Orsini F., Clementi A.E., Lattanzi W., Giardina B., Michetti F. (2008)
  Mitochondrial oxygen consumption inhibition importance for TMT-dependent cell death in undifferentiated PC12 cells
  in Neurochemistry international
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Misiti F., Orsini F., Clementi A.E., Lattanzi W., Giardina B., Michetti F. (literal)

Pagina inizio

• 1092 (literal)

Pagina fine

• 1099 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 52 (literal)

Rivista

• Neurochemistry international (Rivista)

Note

• ISI Web of Science (WOS) (literal)
• PubMed (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a Department of Health and Motor Sciences, University of Cassino, Viale Bonomi, 03043 Cassino (FR), Italy b Institute of Biochemistry and Clinical Biochemistry, Catholic University, Largo F. Vito 1, 00168 Rome, Italy c CNR, Istituto di Chimica del Riconoscimento Molecolare (ICRM), Largo F. Vito 1, 00168 Rome, Italy d Institute of Anatomy and Cell Biology, Catholic University, Largo F. Vito 1, 00168 Rome, Italy (literal)

Titolo

• Mitochondrial oxygen consumption inhibition importance for TMT-dependent cell death in undifferentiated PC12 cells (literal)

Abstract

• The evolving role of mitochondria as a target for different death-inducing noxae prompted us to investigate trimethyltin (TMT)-dependent effects on mitochondrial functionality. For this purpose, we used a homogeneous cell culture model represented by undifferentiated PC12 cells. Mitochondria isolated from PC 12 cells treated with TMT for 6, 12 and 24 h, showed a time-dependent inhibition of ADP-stimulated oxygen consumption using succinate or glutamate/malate as substrate. Using a fluorescent assay, the effect of TMT on mitochondrial membrane potential (Delta Psi) in PC12 cells was also determined. After 24 h in culture, a strong loss of mitochondrial membrane potential (Delta Psi) was observed in TMT-treated cells. Collapse of mitochondrial membrane potential correlated with an increased expression of bax/bcl-2 ratio, as evaluated by polymerase chain reaction. Western blotting and spectrophotometric analysis showed that cytochrome c release and activation of caspase 3 were concurrently induced. Our findings suggest that inhibition of mitochondrial respiration represents the early toxic event for cell death in PC12 due to trimethyltin. (literal)

Prodotto di

• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)
• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)

Autore CNR

• MARIA ELISABETTA CLEMENTI (Unità di personale interno)
• BRUNO GIARDINA (Persona)

Incoming links:

Autore CNR di

• BRUNO GIARDINA (Persona)
• MARIA ELISABETTA CLEMENTI (Unità di personale interno)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Neurochemistry international (Rivista)