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Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis (Articolo in rivista)
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- Label
- Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.ab.2005.05.017 (literal)
- Alternative label
Viktor Víglasky (1); Patrik Danko (1), Jozef Adamcík (1), Francesco Valle (2), Giovanni Dietler (2) (2005)
Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis
in Analytical biochemistry (Print); Elsevier BV, Amsterdam (Paesi Bassi)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Viktor Víglasky (1); Patrik Danko (1), Jozef Adamcík (1), Francesco Valle (2), Giovanni Dietler (2) (literal)
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- http://dx.doi.org/10.1016/j.ab.2005.05.017 (literal)
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- (1) Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safárik University, 04154 Ko?ice, Slovakia
(2) Laboratoire de Physique de la Matière Vivante, IPMC, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland (literal)
- Titolo
- Detection of cruciform extrusion in DNA by temperature-gradient gel electrophoresis (literal)
- Abstract
- Repetitive sequences in DNA molecules, some of which are palindromic, tend to form stable cruciforms. These are frequently located in promoter regions of a specific operon and origin of replication. Temperature gradient gel electrophoresis can be used to distinguish among various supercoiled DNA topoisomers and to ascertain whether or not the cruciform motif has been extruded. In the current study, this technique is implemented for the first time to address the role of temperature in cruciform extrusion from plasmids (literal)
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