Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ultramic.2007.04.010 (literal)

Alternative label

• Melanie Favre (1); Lilia A. Chtcheglova (1); Dimitri A. Lapshin (2); Sergej K. Sekatskii (1); Francesco Valle (1); Giovanni Dietler (1) (2007)
  Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex
  in Ultramicroscopy (Amst.); Elsevier, Amsterdam (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Melanie Favre (1); Lilia A. Chtcheglova (1); Dimitri A. Lapshin (2); Sergej K. Sekatskii (1); Francesco Valle (1); Giovanni Dietler (1) (literal)

Pagina inizio

• 882 (literal)

Pagina fine

• 886 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 107 (literal)

Rivista

• Ultramicroscopy (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 10-11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) Ecole Polytechnique Federale de Lausanne, LPMV (2) Russian Academy of Sciences (literal)

Titolo

• Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex (literal)

Abstract

• We have recently developed a new method for directly measuring the spring constant of single molecules and molecular complexes on a real-time basis [L.A. Chtcheglova, G.T. Shubeita, S.K. Sekatskii, G. Dietler, Biophys. J. 86 (2004) 1177]. The technique combines standard force spectroscopy with a small dithering of tip. Changes in the amplitude of the oscillations are measured as a function of the pulling-off force to yield the spring constant of the complex. In this report, we present the first results of combination of this approach with the force-clamp spectroscopy. The standard atomic-force microscope has been supplemented with an electronic unit, which is capable of realizing an arbitrary force function, and permits the force-loading regime to be interrupted at any time. Using this method, the time needed to rupture a single bond can be measured as a function of the force that is required to maintain the complex in a stretched condition. The energy landscape of the avidin-biotin complex is explored and discussed. (literal)

Editore

• Elsevier (Editore)

Prodotto di

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)
• Nanotecnologie di materiali multifunzionali e sistemi biologici (PM.P05.010.001) (Modulo)

Autore CNR

• FRANCESCO VALLE (Persona)

Insieme di parole chiave

• Parole chiave di "Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per lo studio dei materiali nanostrutturati (ISMN) (Istituto)
• Nanotecnologie di materiali multifunzionali e sistemi biologici (PM.P05.010.001) (Modulo)

Autore CNR di

• FRANCESCO VALLE (Persona)

Editore di

• Elsevier (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Ultramicroscopy (Rivista)

Insieme di parole chiave di

• Parole chiave di "Force-clamp spectroscopy with a small dithering of AFM tip, and its application to explore the energy landscape of single avidin-biotin complex" (Insieme di parole chiave)