Short communication: Predominance of β-casein (CSN2) C allele in goat breeds reared in Italy (Articolo in rivista)

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  • Short communication: Predominance of β-casein (CSN2) C allele in goat breeds reared in Italy (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Stefania Chessa; Elena Budelli; Francesca Chiatti; Anna Maria Cito; Patrizia Bolla; Anna Caroli (2005)
    Short communication: Predominance of β-casein (CSN2) C allele in goat breeds reared in Italy
    in Journal of dairy science; American Dairy Science Association-ADSA, Champaign (Stati Uniti d'America)
    (literal)
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  • Stefania Chessa; Elena Budelli; Francesca Chiatti; Anna Maria Cito; Patrizia Bolla; Anna Caroli (literal)
Pagina inizio
  • 1878 (literal)
Pagina fine
  • 1881 (literal)
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  • 88 (literal)
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  • 4 (literal)
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  • 5 (literal)
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  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
  • PubMe (literal)
  • Google Scholar (literal)
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  • Stefania Chessa, Francesca Chiatti, Patrizia Bolla - Dipartimento di Scienze e Tecnologie Veterinarie Per la Sicurezza Alimentare, Universit√† degli Studi di Milano, 20134 Milano, Italy; Elena Budelli - Fondazione Parco Tecnologico Padano, Centro Ricerche e Studi Agroalimentari, 20090 Segrate (Milano), Italy; Anna Maria Cito, Anna Caroli - Dipartimento di Sanit√† e Benessere Animale, Universit√† di Bari, 70010 Valenzano (Bari), Italy (literal)
Titolo
  • Short communication: Predominance of β-casein (CSN2) C allele in goat breeds reared in Italy (literal)
Abstract
  • A protocol for the rapid and simultaneous genotyping of A, C, and 0' CSN2 alleles in goat was developed by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) technique. Screening the CSN2 variability in 7 goat breeds reared in Italy validated the genotyping test. The SSCP-PCR technique was also suitable for monitoring CSN2 polymorphism. In particular, the discrimination between CSN2*A and CSN2*C is important because the 2 corresponding protein variants cannot be separated by standard typing techniques. The monitoring of CSN2 variability in the goat breeds indicates the predominance of the C allele. In most breeds, CSN2*C occurred with the highest frequency, except in Saanen where CSN2*A and CSN2*C showed similar frequencies. Variant CSN2*C occurred with a frequency of 0.68 (Camosciata), 0.70 (Jonica), 0.71 (Garganica), 0.82 (Maltese), 0.87 (Cilentana), and 0.97 (Orobica). The alignment among the mature CSN2 sequences of different species suggests that CSN2*A is the ancestral allele compared with CSN2*C. Interestingly, the CSN2*A goat variant showed higher frequencies in selected breeds (Saanen and Camosciata). (literal)
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