http://www.cnr.it/ontology/cnr/individuo/prodotto/ID175818

Technical note: Simultaneous identification of CSN1S2 A, B, C, and E alleles in goats by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista)

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Technical note: Simultaneous identification of CSN1S2 A, B, C, and E alleles in goats by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista) (literal)

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Alternative label

Stefania Chessa; Daniela Rignanese; Francesca Chiatti; Annalisa Radeghieri; Carmen Gigliotti; Anna Caroli (2008)
Technical note: Simultaneous identification of CSN1S2 A, B, C, and E alleles in goats by polymerase chain reaction-single strand conformation polymorphism
in Journal of dairy science; American Dairy Science Association-ADSA, Champaign (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Stefania Chessa; Daniela Rignanese; Francesca Chiatti; Annalisa Radeghieri; Carmen Gigliotti; Anna Caroli (literal)

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Note

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Stefania Chessa, Daniela Rignanese, Francesca Chiatti - Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Universita degli Studi di Milano, Via Trentacoste 2, Milano 20134, Italy; Annalisa Radeghieri, Carmen Gigliotti, Anna Caroli - Dipartimento di Scienze Biomediche e Biotecnologie, Universita degli Studi di Brescia, Viale Europa 11, Brescia 25123, Italy (literal)

Titolo

Technical note: Simultaneous identification of CSN1S2 A, B, C, and E alleles in goats by polymerase chain reaction-single strand conformation polymorphism (literal)

Abstract

Most variability in goat caseins originates from the high number of genetic polymorphisms often affecting the specific protein expression, with strong effects on milk composition traits and technological properties. At least 7 alleles have been found in the goat ? S2-CN gene (CSN1S2). Five of them (CSN1S2*A, CSN1S2*B, CSN1S2*C, CSN1S2*E, and CSN1S2*F) are widespread in most breeds, whereas the other 2 (CSN1S2*D and CSN1S2*0) are rarer alleles. Four different PCR-RFLP tests are needed to detect all of these variants at the DNA level. The objective of this study was to develop and validate a rapid method for typing 4 of the 5 most-common goat CSN1S2 alleles by means of PCR-single strand conformation polymorphism (SSCP). The method was validated by analyzing 37 goat samples at the protein and DNA level, respectively, by milk isoelectrofocusing and PCR-RFLP methods already described. The genotypes obtained using the PCR-SSCP approach were in full agreement with those obtained by the validation analyses. The newly developed PCR-SSCP approach provides an accurate and inexpensive assay highly suitable for genotyping goat CSN1S2. (literal)

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