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Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista)

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Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista) (literal)

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Gabriella Ceriotti; Stefania Chessa; Patrizia Bolla; Elena Budelli; Leonardo Bianchi; Emilia Duranti; Anna Caroli (2004)
Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism
in Journal of dairy science; American Dairy Science Association-ADSA, Champaign (Stati Uniti d'America)
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Gabriella Ceriotti; Stefania Chessa; Patrizia Bolla; Elena Budelli; Leonardo Bianchi; Emilia Duranti; Anna Caroli (literal)

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Gabriella Ceriotti, Stefania Chessa, Patrizia Bolla - Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, 20134 Milano, Italy; Elena Budelli - Fondazione Parco Tecnologico Padano, Centro Ricerche e Studi Agroalimentari, 20090 Segrate (Milano), Italy; Leonardo Bianchi, Emilia Duranti - Dipartimento di Scienze Zootecniche, Universita` degli Studi di Perugia, 06100 Perugia, Italy; Anna Caroli - Dipartimento di Sanità e Benessere Animale, Università di Bari, 70010 Valenzano (Bari), Italy (literal)

Titolo

Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism (literal)

Abstract

Casein genetic polymorphisms are important and well known due to their effects on quantitative traits and technological properties of milk. At the DNA level, polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) allows for the simultaneous typing of several alleles at casein loci, as well as the detection of unknown polymorphisms. Here we describe the usefulness of the PCR-SSCP technique for casein typing in sheep. In particular, three single-nucleotide polymorphisms (SNP) are described at CSN1S1, CSN2, and CSN3, all resulting in amino acid exchanges. At CSN1S1, a transition T -> C was found, resulting in the deduced amino acid exchange Ile186 -> Thr186. A transition A -> G resulting in the deduced amino acid exchange Met183 -> Val183 was identified at CSN2. The 2 SNP showed a rather high frequency (ranging from 0.12 to 0.26) in 3 Italian breeds (Sarda, Comisana, Sopravissana). Another transition C -> T (Ser 104 -> Leu104) was found at CSN3 in one heterozygous animal. (literal)

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