Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.3168/jds.S0022-0302(03)73978-2 (literal)

Alternative label

• Stefania Chessa; Elena Budelli; Katja Gutscher; Anna Caroli; Georg Erhardt (2003)
  Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism
  in Journal of dairy science; American Dairy Science Association-ADSA, Champaign (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Stefania Chessa; Elena Budelli; Katja Gutscher; Anna Caroli; Georg Erhardt (literal)

Pagina inizio

• 3726 (literal)

Pagina fine

• 3729 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 86 (literal)

Rivista

• Journal of dairy science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 4 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopus (literal)
• PubMe (literal)
• Google Scholar (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Stefania Chessa - Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, 20134 Milano, Italy; Elena Budelli - Fondazione Parco Tecnologico Padano, Centro Ricerche e Studi Agroalimentari, 20090 Segrate (Milano), Italy; Katja Gutscher, Georg Erhardt - Institut für Tierzucht und Haustiergenetik, Justus-Liebig Universität, 35390 Gie?en, Germany; Anna Caroli - Dipartimento di Sanità e Benessere Animale, Università di Bari, 70010 Valenzano (Bari), Italy (literal)

Titolo

• Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism (literal)

Abstract

• Until now, a total of nine polymorphic sites corresponding to six different alleles have been described at the ?-casein (CSN3) locus in the domestic goat (Capra hircus). A protocol for the rapid and simultaneous genotyping of five goat CSN3 alleles by using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique was developed. Moreover, the developed test was validated by screening the CSN3 variability in four Italian breeds, Garganica, Jonica, Maltese, and Camosciata. Seven different patterns were readily identifiable. These corresponded to five known alleles and two newly identified variants. The G/A substitution at nucleotide position 471, which is not identifiable at the protein level but was found to be very frequent in the typed breeds, is easily detectable by the protocol developed. The PCR-SSCP analysis is a powerful tool for the genetic study of CSN3 variability in domestic goats, allowing both the simultaneous identification of different alleles, and the detection of new variants. (literal)

Editore

• American Dairy Science Association-ADSA (Editore)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR

• STEFANIA CHESSA (Persona)

Insieme di parole chiave

• Keywords of "Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR di

• STEFANIA CHESSA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of dairy science (Rivista)

Editore di

• American Dairy Science Association-ADSA (Editore)

Insieme di parole chiave di

• Keywords of "Short communication: Simultaneous identification of five kappa-casein (CSN3) alleles in domestic goat by polymerase chain reaction-single strand conformation polymorphism" (Insieme di parole chiave)