http://www.cnr.it/ontology/cnr/individuo/prodotto/ID17566
Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma (Articolo in rivista)
- Type
- Label
- Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Desiderio C.; De Rossi A.; Inzitari R.; Mancinelli A.; Rossetti D.V.; Castagnola M.; Messana I. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- C. Desiderio: Istituto di Chimica del Riconoscimento Molecolare-Sez di Roma,Consiglio Nazionale delle Ricerche,c/o Istituto di Biochimica e Biochimica Clinica dell'Università Cattolica del Sacro Cuore,L.go F. Vito 1,00168 Roma, Italy;
A. De Rossi: Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche,Area della Ricerca Roma 1, Via Salaria km 29.300,00016 Monterotondo Stazione,Roma, Italy;
R. Inzitari, D. V. Rossetti, Castagnola: Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore,L.go F. Vito 1,00168 Roma, Italy;
A. Mancinelli: Dipartimento Scientifico SIGMA-TAU,Via Pontina km 30,400,00040 Pomezia,Roma, Italy;
I. Messana: Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari,Campus Monserrato,09042 Monserrato,Cagliari, Italy (literal)
- Titolo
- Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma (literal)
- Abstract
- A capillary electrophoresis (CE) method coupled
to electrospray ionization ion trap tandem mass spectrometry
(ESI-IT-MS/MS) is described for the rapid analysis of
carnitine, acetylcarnitine, and propionylcarnitine in human
plasma. Optimization of the procedure was achieved by a
reduced sample pretreatment and after examining several
physicochemical parameters that influence both the CE
separation and the MS analytes detection. The analysis of
total carnitine in human plasma after hydrolysis of shortchain
metabolites is also shown. The analysis of carnitine
and metabolites was obtained in less than 10 min using a
200 mM ammonium formate buffer, pH 2.5, with high
sensitivity and specificity using the MS detection in product
ion scan mode. The method was tested for quantitative
recovery using dialyzed human plasma as matrix and
showed linearity in the concentrations ranges 20-160, 1-
32, and 0.25-8 ?M for carnitine, acetylcarnitine, and
propionylcarnitine with (squared) correlation coefficients
of 0.9984, 0.9995, and 0.9991, respectively. The intraday
and intermediate analysis repeatability and accuracy are
within 15% of relative standard deviation (RSD) at low,
medium, and high concentration and within/or slight
exceeding 20% at the lower limit of quantitation (LLOQ).
The method is sensitive for determining carnitine and its
metabolites in human plasma with high specificity. (literal)
- Prodotto di
- Autore CNR
- Insieme di parole chiave
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
- Insieme di parole chiave di