http://www.cnr.it/ontology/cnr/individuo/prodotto/ID175400

Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures (Articolo in rivista) (literal)

Anno

2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.bios.2004.06.026 (literal)

Alternative label

Curulli A.; Valentini F.; Orlanduci S.; Terranova M. L.; Palleschi G. (2004)
Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures
in Biosensors & bioelectronics; Elsevier Science London, London (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Curulli A.; Valentini F.; Orlanduci S.; Terranova M. L.; Palleschi G. (literal)

Pagina inizio

1223 (literal)

Pagina fine

1232 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.sciencedirect.com/science/article/pii/S0956566304002751 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

20 (literal)

Rivista

Biosensors & bioelectronics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

10 (literal)

Note

ISI Web of Science (WOS) (literal)
Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

CNR ISMN Istituto per lo Studio dei Materiali Nanostrutturati Sezione Roma 2, Via del Castro Laurenziano, 700161 Roma, Italy Dipartimento di Scienze e Tecnologie Chimiche, II Università di Roma, Tor Vergata, Viale della Ricerca Scientifica, 00133 Roma, Italy (literal)

Titolo

Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures (literal)

Abstract

Conductive polymer nanotubules of 1,2-diaminobenzene (1,2-DAB) were prepared using a porous polycarbonate membrane template, placed on a Pt foil and used to support the polymer, then, the electropolymerisation was performed by chronocoulometry. The obtained conductive polymer nanostructures were then placed on Pt electrode and used to support highly dispersed prussian blue (PB), which acts as the active component for H2O2 detection. The observed good stability of PB as catalyst of H2O2 was related to the presence of organic non-conventional conducting polymers in a composite nanostructured film. These nanostructured polymer/PB composite films were also characterised by scanning electron microscopy (SEM) and Raman spectroscopy. The non-conventional conducting polymer nanotubules/PB modified Pt electrodes were tested by cyclic voltammeter for stability at different pH values, then, by amperometry, for hydrogen peroxide, ascorbic acid, acetaminophen, uric acid and acetylcholine. Glucose oxidase (GOD), lactate oxidase (LOD), l-aminoacid oxidase (l-AAOD), alcohol oxidase (AOD), glycerol-3-phosphate oxidase (GPO), lysine oxidase (LyOx), and choline oxidase (ChOx) were immobilised on PB layer supported on 1,2-diaminobenzene (1,2-DAB) nanotubules onto the Pt electrodes. Different strategies for enzyme immobilisation were performed and used. Analytical parameters such as reproducibility, interference rejection, response time, storage and operational stability of the sensors have been studied and optimised. Results provide a guide to design high sensitive, stable and interference-free biosensors. The glucose biosensors assembled with nanostructured poly(1,2-DAB) showed a detection limit of 5 × 10-5 mol l-1, a wide linearity range (5 × 10-5 to 5 × 10-3 mol l-1), a high selectivity, a stability of 3 months at 4 oC, and at least 4 weeks at room temperature. Similar analytical parameters and stability were also studied for l-(+)-lactic acid, l-leucine, ethanol, glycerol-3-phosphate, lysine, and choline biosensors. (literal)

Editore