Functional and structural characterization of the myoglobin from the polychaete Ophelia bicornis (Articolo in rivista)

Type
Label
  • Functional and structural characterization of the myoglobin from the polychaete Ophelia bicornis (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1042/BJ20050267 (literal)
Alternative label
  • Sanna M.T., Manconi B., Castagnola M., Giardina B., Masia D., Messana I., Olianas A., Patamia M., Petruzzelli R., Pellegrini M. (2005)
    Functional and structural characterization of the myoglobin from the polychaete Ophelia bicornis
    in Biochemical journal (Lond., 1984)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sanna M.T., Manconi B., Castagnola M., Giardina B., Masia D., Messana I., Olianas A., Patamia M., Petruzzelli R., Pellegrini M. (literal)
Pagina inizio
  • 497 (literal)
Pagina fine
  • 505 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 389 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 9 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • M. Teresa SANNA*1, Barbara MANCONI*, Massimo CASTAGNOLA+?, Bruno GIARDINA+?, Daniela MASIA*, Irene MESSANA*?, Alessandra OLIANAS*, Maria PATAMIA?, Raffaele PETRUZZELLI┬ž and Mariagiuseppina PELLEGRINI* *Dipartimento di Scienze Applicate ai Biosistemi, Universit`a di Cagliari, Cittadella Universitaria, I-09042 Monserrato (CA), Italy, +Istituto di Biochimica e Biochimica Clinica, Facolt`a di Medicina, Universit`a Cattolica, Rome, Italy, ?Istituto per la Chimica del Riconoscimento Molecolare, CNR, Largo F. Vito 1, I-00168, Rome, Italy, and ┬žDipartimento di Scienze Biomediche, Universit`a \"G. D' Annunzio\", I-66100 Chieti, Italy The (literal)
Titolo
  • Functional and structural characterization of the myoglobin from the polychaete Ophelia bicornis (literal)
Abstract
  • The myoglobin of the polychaete annelid Ophelia bicornis was isolated, purified to homogeneity and characterized. The primary structure, obtained from cDNA and protein sequencing, consists of 139 amino acid residues. The alignment with other globin sequences showed that O. bicornis myoglobin misses the pre-A helix and the first six residues of the A helix. The presence of a PheB10-GlnE7 haem distal residue pair is in agreement with the measured oxygen affinity (P-50 = 0.85 mmHg; 1 mmHg = 0.133 kPa) and the only slightly higher autoxidation rate constant (0.28 h(-1)) with respect to that of the sperm whale myoglobin mutant E7 His -> Gln (0.21 h(-1)) and to elephant myoglobin (0.1 h(-1)). Oxygen-binding co-operativity was found to be absent under all the examined experimental conditions. The resistance of O. bicornis myoglobin towards autoxidation seems to confirm the important role of part of the A helix in the stability of the globin. The higher pK, of the acid-alkaline ferric transition of O. bicornis with respect to Asian elephant myoglobin, as well as the higher absorbance ratio of its ferric form to the oxy form measured in the Soret region (gamma(met)/gamma(oxy)) with respect to that of the African elephant myoglobin, suggested a stronger interaction between the distal glutamine and the water molecule at the sixth co-ordinate position. (literal)
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