Membrane assisted coupled enzyme system for phospholipid modification (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Membrane assisted coupled enzyme system for phospholipid modification (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• D'Arrigo P., Fasoli E., Pedrocchi-Fantoni G., Servi S., Tessaro D. (2005)
  Membrane assisted coupled enzyme system for phospholipid modification
  in Enzyme and microbial technology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• D'Arrigo P., Fasoli E., Pedrocchi-Fantoni G., Servi S., Tessaro D. (literal)

Pagina inizio

• 435 (literal)

Pagina fine

• 440 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 37 (literal)

Rivista

• Enzyme and microbial technology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• ICRM, CNR (literal)

Titolo

• Membrane assisted coupled enzyme system for phospholipid modification (literal)

Abstract

• Phospholipase D obtained from a culture of Streptomyces sp. strain PMF, was immobilised in a hollow fiber membrane by ultrafiltration. The reactor was used to contact an organic solution containing phosphatidylcholine (PC) and a water phase; the rate of formation of the hydrolysis product (phosphatidic acid, PA) depending on different parameters (pH, temperature, PC concentration and enzyme units) was measured. Subsequently, the water phase was replaced with a 2-3 M solution of different alcohols (L-serine, glycerol and ethanolamine) and the rate of transphosphatidylation was studied and finally optimized using glycerol as model alcohol. The ratio of formation of phosphatidylglycerol (PG) (transphosphatidylation reaction) over PA (hydrolysis reaction) was minimized working at pH 4.5 where hydrolysis rate is lower. The operational stability of the system was excellent during a period of several months. Purification of the reaction mixture from PA was effected by selectively hydrolysing it with phosphatase (Pase). Co-immobilization of PLD and Pase resulted in effective PA removal. Although the space-time yield of the transphosphatidylation reaction is lower than the one observed in a biphasic system, product purification is unnecessary, product isolation easier and enzyme stability higher. (literal)

Prodotto di

• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)
• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)

Autore CNR

• STEFANO SERVI (Unità di personale esterno)
• GIUSEPPE ANDREA PEDROCCHI FANTONI (Persona)

Incoming links:

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Modellistica molecolare di proteine legate a processi patologici (PM.P01.026.002) (Modulo)
• Applicazioni sintetiche della biocatalisi (PM.P03.009.002) (Modulo)

Autore CNR di

• GIUSEPPE ANDREA PEDROCCHI FANTONI (Persona)
• STEFANO SERVI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Enzyme and microbial technology (Rivista)