The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis (Articolo in rivista)

Type
Label
  • The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1073/pnas.0812789106 (literal)
Alternative label
  • Grimaldi P.; Orlando P.; Di Siena S.; Lolicato F.; Petrosino S.; Bisogno T.; Geremia R.; De Petrocellis L. (2009)
    The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis
    in Proceedings of the National Academy of Sciences of the United States of America
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Grimaldi P.; Orlando P.; Di Siena S.; Lolicato F.; Petrosino S.; Bisogno T.; Geremia R.; De Petrocellis L. (literal)
Pagina inizio
  • 11131 (literal)
Pagina fine
  • 11136 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 106 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 27 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Public Health and Cellular Biology, University of Rome “Tor Vergata”, 00133 Rome, Italy; Institute of Protein Biochemistry (PO), Consiglio Nazionale delle Ricerche, via P. Castellino 111, Napoli, Italy; Institutes of Biomolecular Chemistry and Cybernetics Consiglio Nazionale delle Ricerche, via Campi Flegrei 34, Pozzuoli, Italy (literal)
Titolo
  • The endocannabinoid system and pivotal role of the CB2 receptor in mouse spermatogenesis (literal)
Abstract
  • The exact role of the endocannabinoid system (ECS) during spermatogenesis has not been clarified. We used purified germ cell fractions representative of all phases of spermatogenesis and primary cultures of spermatogonia. This approach allowed the precise quantification of the cannabinoid receptor ligands, anandamide and 2-arachidonoylglycerol, and of the expression at transcriptional and transductional levels of their metabolic enzymes and receptors. Our data indicate that male mouse germ cells possess an active and complete ECS, which is modulated during meiosis, and suggest the presence of an autocrine endocannabinoid signal during spermatogenesis. Mitotic cells possess higher levels of 2-arachidonoylglycerol, which decrease in spermatocytes and spermatids. Accordingly, spermatogonia express higher and lower levels of 2-arachidonoylglycerol biosynthetic and degrading enzymes, respectively, as compared to meiotic and postmeiotic cells. This endocannabinoid likely plays a pivotal role in promoting the meiotic progression of germ cells by activating CB(2) receptors. In fact, we found that the selective CB(2) receptor agonist, JWH133, induced the Erk 1/2 MAPK phosphorylation cascade in spermatogonia and their progression toward meiosis, because it increased the number of cells positive for SCP3, a marker of meiotic prophase, and the expression of early meiotic prophase genes. (literal)
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