Acrylamide-agarose copolymers: Improved resolution of high molecular mass proteins in two-dimensional gel electrophoresis (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Acrylamide-agarose copolymers: Improved resolution of high molecular mass proteins in two-dimensional gel electrophoresis (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Roncada P., Cretich M., Fortin R., Agosti S., De Franceschi L., Greppi G.F., Turrini F., Carta F., Turri S., Levi M., Chiari M. (2005)
  Acrylamide-agarose copolymers: Improved resolution of high molecular mass proteins in two-dimensional gel electrophoresis
  in Proteomics (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Roncada P., Cretich M., Fortin R., Agosti S., De Franceschi L., Greppi G.F., Turrini F., Carta F., Turri S., Levi M., Chiari M. (literal)

Pagina inizio

• 2331 (literal)

Pagina fine

• 2339 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• Proteomics (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Acrylamide-agarose copolymers: Improved resolution of high molecular mass proteins in two-dimensional gel electrophoresis (literal)

Abstract

• A method was developed in order to analyse high molecular mass proteins by two-dimensional (2-D) electrophoresis using a copolymer of acrylamide and allyl agarose instead of Bis cross-linked polyacrylamide (PA) gels in sodium dodecyl sulphate-electrophoresis. In this work, the matrix composition was optimised to improve the resolution of proteins larger than 200 kDa. The new gel type does not entrap large proteins and protein complexes at the application site. Mechanical properties were investigated through rheological measurements, which suggested the formation of a highly entangled elastomeric soft gel. A high 2-D resolution of proteins, extracted from membranes of red blood cells, was obtained in these gels. An example of tryptic digestion, peptide extraction and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry was reported. The results demonstrate that the new gel is fully compatible with mass spectrometry protein analysis. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Sintesi di materiali funzionali e sviluppo di sistemi analitici miniaturizzati (PM.P06.003.001) (Modulo)

Autore CNR

• MARINA CRETICH (Persona)
• MARCELLA CHIARI (Unità di personale interno)

Incoming links:

Autore CNR di

• MARINA CRETICH (Persona)
• MARCELLA CHIARI (Unità di personale interno)

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Sintesi di materiali funzionali e sviluppo di sistemi analitici miniaturizzati (PM.P06.003.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proteomics (Rivista)