http://www.cnr.it/ontology/cnr/individuo/prodotto/ID173344
N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation (Articolo in rivista)
- Type
- Label
- N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Nocca G; D'Antò V; Desiderio C; Rossetti DV; Valletta R; Baquala AM; Schweikl H; Lupi A; Rengo S; Spagnuolo G (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Giuseppina Nocca (a), Vincenzo D'Anto` (b), Claudia Desiderio (c), Diana Valeria Rossetti (a), Rosa Valletta (b),
Adriana Marquez Baquala (a), Helmut Schweikl (d), Alessandro Lupi (c), Sandro Rengo (b), Gianrico Spagnuolo (b),
(a) Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy
(b) Department of Oral and Maxillofacial Sciences, University of Naples ''Federico II'', Napoli, Italy
(c) Molecular Recognition Chemistry Institute, C.N.R, Rome, Italy
(d) Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany (literal)
- Titolo
- N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation (literal)
- Abstract
- Cytotoxicity of the dental resin monomer 2-hydroxyethyl methacrylate (HEMA) and the protective effects
of N-acetyl cysteine (NAC) on monomer-induced cell damage are well demonstrated. The aim of our study
was to analyze the hypothesis that the protection of NAC from HEMA cytotoxicity might be due to direct
NAC adduct formation. To this end, using HPLC we first measured the actual intracellular HEMA concentrations
able to cause toxic effects on 3T3-fibroblasts and then determined the decrease in intracellular and
extracellular HEMA levels in the presence of NAC. In addition, by capillary electrophoresis coupled with
mass spectrometry analysis (CE-MS), we evaluated NAC-HEMA adduct formation. HEMA reduced 3T3 cell
vitality in a dose- and time-dependent manner. The concentration of HEMA inside the cells was 15-20
times lower than that added to the culture medium for cell treatment (0-8 mmol/L). In the presence of
10 mmol/L NAC, both intracellular and extracellular HEMA concentrations greatly decreased in conjunction
with cytotoxicity. NAC-HEMA adducts were detected both in the presence and absence of cells. Our
findings suggest that the in vitro detoxification ability of NAC against HEMA-induced cell damage occurs
through NAC adduct formation. Moreover, we provide evidence that the actual intracellular concentration
of HEMA able to cause cytotoxic effects is at least one magnitude lower than that applied extracellularly. (literal)
- Prodotto di
- Autore CNR
- Insieme di parole chiave
Incoming links:
- Prodotto
- Autore CNR di
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
- Insieme di parole chiave di