http://www.cnr.it/ontology/cnr/individuo/prodotto/ID173297
Nonenzymatic Ligation of an RNA Oligonucleotide Analyzed by Atomic Force Microscopy (Articolo in rivista)
- Type
- Label
- Nonenzymatic Ligation of an RNA Oligonucleotide Analyzed by Atomic Force Microscopy (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Alternative label
Pino S, Biasiucci M, Scardamaglia M, Gigli G, Betti MG, Mariani C, Di Mauro E (2011)
Nonenzymatic Ligation of an RNA Oligonucleotide Analyzed by Atomic Force Microscopy
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Pino S, Biasiucci M, Scardamaglia M, Gigli G, Betti MG, Mariani C, Di Mauro E (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- DOI: 10.1021/jp200832w (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Department of Physics and Department of Biology and Biotechnologies Charles Darwin, Sapienza University of Rome, P.le A. Moro 2, 00185 Rome, Italy
NNL-CNR Nanoscience Institute, Via Arnesano, 73100 Lecce, Italy
Electronical Engineering Department, University of Rome Tor Vergata, Via del Politecnico, 00133 Rome, Italy
Innovation Engineering Department, University of Salento, via Arnesano, 73100 Lecce, Italy
Nanotechnology and Nanoscience Laboratory, Research Center on Nanotechnology Applied to Engineering, CNIS, Sapienza University of Rome, Italy (literal)
- Titolo
- Nonenzymatic Ligation of an RNA Oligonucleotide Analyzed by Atomic Force Microscopy (literal)
- Abstract
- The products of ligation reaction of a 24 nucleotides long PolyA RNA adsorbed on mica were observed by atomic force microscopy. The occurrence of oligonucleotides at different degrees of polymerization has been quantitatively studied before and after ligation reaction. The microscopy images at the nanoscale show that nonenzymatic ligation of pristine RNA monomers results in the formation of supramolecular aggregates, with prevalence of dimers and tetramers. Analytical conditions were defined allowing the identification, the quantitative evaluation, and their distribution after ligation reaction, also providing an estimate of the degree of hydration of the objects. Such investigation is of particular biological relevance and provides the simplest yet model system for direct investigation of RNA reactions by advanced microscopy. (literal)
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