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The influence of polymeric membrane surface free energy on cell metabolic functions (Articolo in rivista)

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The influence of polymeric membrane surface free energy on cell metabolic functions (Articolo in rivista) (literal)

Anno

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De Bartolo L. 1, Morelli S. 1, Bader A. 2,3 and Drioli E. 1 (2001)
The influence of polymeric membrane surface free energy on cell metabolic functions
in Journal of materials science. Materials in medicine
(literal)

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Publication on Journal of Materials Science: Materials in Medicine that is an established refereed monthly companion to the Journal of Materials Science. It publishes papers on the science and technology of biomaterials. It is the official Journal of the European Society for Biomaterials (literal)

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Rivista

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This study was performed to understand the role of surface free energy of different polymeric membranes in the interaction with liver cells. We compared cell metabolic functions on membranes with different surface free energy as parameters of cytocompatibility. Since serum proteins modify membrane surface properties, we tested the surface free energy of membranes before and after modification into culture medium containing serum proteins. The obtained results showed that the cell-membrane interaction of the investigated membranes is improved by changing its surface free energy. The membrane surface free energy was modified by protein adsorption. Maximum protein adsorption occurs on membranes with lowest surface free energy, corresponding at the most hydrophobic. For all investigated membranes, a rise in the surface free energy promoted liver specific functions of cells. Enhancement of the ammonia elimination rate is related to the membrane surface free energy. Our results suggest that it is possible to improve the cytocompatibility of the membrane surface by changing its physico-chemical surface characteristics. However, the surface free energy is only one of the indicators of cell behaviour on membranes. Other surface parameters such as surface charge or the chemical structure of a polymer can also have an effect on the overall behaviour of tissue on polymeric membranes. (literal)

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Affiliazione autori: (max 500 caratteri) 1) ITM-CNR; 2)Leibniz Institute for Biotechnology and Artificial Organs (LEBAO) Hannover Germany; 3)Gesellschaft für Biotechnologische Forschung Braunschweig (GBF), Braunschweig, Germany (literal)

Titolo

The influence of polymeric membrane surface free energy on cell metabolic functions (literal)

Abstract

In membrane bioartificial organs using isolated cells, polymeric semipermeable membranes are used as immunoselective barriers, means for cell oxygenation and also as substrata for adhesion of anchorage-dependent cells. The selection of cytocompatible membranes that promote in vitro cell adhesion and function could be dependent on its membrane properties. In this study we investigated the physicochemical aspects of the interaction between the membrane and mammalian cells in order to provide guidelines to the selection of cytocompatible membranes. We evaluated the metabolic behaviour of isolated liver cells cultured on various polymeric membranes such as the ones modified by protein adsorption. The physico-chemical properties of the membranes were characterised by contact angle measurements. The surface free energy of membranes and their different parameters acid (×+), base (×-) and Lifshitz-van der Waals (×LW) were calculated according to Good-van Oss¡¦s model. The adsorption of protein modified markedly both contact angle and membrane surface tension. In particular, membrane surface free energy decreased drastically with increased water contact angle. For each investigated membrane we observed that liver specific functions of cells improve on hydrophilic membrane surfaces. For all investigated membranes the rate of ammonia elimination increased with increasing of membrane surface free energy. (literal)

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