2D-electrophoretic analysis for studying the tolerance to heavy metals in Rhodobacter sphaeroides (Comunicazione a convegno)

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  • 2D-electrophoretic analysis for studying the tolerance to heavy metals in Rhodobacter sphaeroides (Comunicazione a convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Francesco Pisani, Luigi R. Ceci, Francesca De Leo, Raffaele Gallerani, Francesca Italiano, Angela Agostiano, Francesco Milano, Massimo Trotta, Alessandro Buccolieri, Livia Giotta (2006)
    2D-electrophoretic analysis for studying the tolerance to heavy metals in Rhodobacter sphaeroides
    in Biotechnology National Congress (CNB9) : Translational Models in Biotechnology, Torino, 7-9 settembre
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Francesco Pisani, Luigi R. Ceci, Francesca De Leo, Raffaele Gallerani, Francesca Italiano, Angela Agostiano, Francesco Milano, Massimo Trotta, Alessandro Buccolieri, Livia Giotta (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • The influence of metal ions on the photosynthetic growth of the facultative anaerobic bacterium Rhodobacter sphaeroides strain R-26.1 is presented. The ability of this bacterial strain to grow photosynthetically in highly contaminated environments was tested in liquid anaerobic media containing transition metal cations Hg2+, Cu2+, Fe2+, Ni2+, Co2+ and oxyanions, such as MoO42- and CrO42-. Growth analysis of Rhodobacter sphaeroides incubated with cobalt and chromate shows a light reduction of growth ability but a relevant cobalt and chromate bioaccumulation [1].This relationship is fundamental in bioremediation of heavy metal polluted sites. The tolerance towards Co2+ of this bacterial species appears particularly high compared to that of other micro-organisms. Preliminary data suggest a role of small soluble proteins in the resistance mechanism to Co2+ exposure.To elucidate the effect of Co2+ on the enzymes involved in tolerance response, a proteomic approach was chosen. Using bidimensional electrophoresis, maps of soluble proteins from R. Sphaeroides R-26.1, grown with and without Co2+ (5mM), have been produced. Many differences can be identified between R. sphaeroides proteomes from control cells and from cells incubated with 5mM Co2+. We have chosen as significative all the spots having a t-Student value ranging between 5 and 19. In this range 53 spots has been identified which will be further analysed by peptide mass fingerprinting. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • (a) Dipartimento di Chimica - Università di Bari. (b ) Istituto per i processi chimico fisici (CNR) sezione di Bari. (c) Dipartimento di scienza dei materiali università di Lecce. (d) Dipartimento di Biochimica e Biologia molecolare Università degli studi di Bari. (e) Istituto di Biomembrane e Bioenergetica (CNR), sezione di Trani. (literal)
Titolo
  • 2D-electrophoretic analysis for studying the tolerance to heavy metals in Rhodobacter sphaeroides (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 88-6136-000-9 (literal)
Abstract
  • The influence of metal ions on the photosynthetic growth of the facultative anaerobic bacterium Rhodobacter sphaeroides strain R-26.1 is presented. The ability of this bacterial strain to grow photosynthetically in highly contaminated environments was tested in liquid anaerobic media containing transition metal cations Hg2+, Cu2+, Fe2+, Ni2+, Co2+ and oxyanions, such as MoO42- and CrO42-. Growth analysis of Rhodobacter sphaeroides incubated with cobalt and chromate shows a light reduction of growth ability but a relevant cobalt and chromate bioaccumulation [1].This relationship is fundamental in bioremediation of heavy metal polluted sites. The tolerance towards Co2+ of this bacterial species appears particularly high compared to that of other micro-organisms. Preliminary data suggest a role of small soluble proteins in the resistance mechanism to Co2+ exposure.To elucidate the effect of Co2+ on the enzymes involved in tolerance response, a proteomic approach was chosen. Using bidimensional electrophoresis, maps of soluble proteins from R. Sphaeroides R-26.1, grown with and without Co2+ (5mM), have been produced. Many differences can be identified between R. sphaeroides proteomes from control cells and from cells incubated with 5mM Co2+. We have chosen as significative all the spots having a t-Student value ranging between 5 and 19. In this range 53 spots has been identified which will be further analysed by peptide mass fingerprinting. (literal)
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