Traffic of botulinum toxins A and E in excitatory and inhibitory neurons. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Traffic of botulinum toxins A and E in excitatory and inhibitory neurons. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Verderio C, Grumelli C, Raiteri L, Coco S, Paluzzi S, Caccin P, Rossetto O, Bonanno G, Montecucco C, Matteoli M. (2007)
  Traffic of botulinum toxins A and E in excitatory and inhibitory neurons.
  in Traffic (Copenhagen. Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Verderio C, Grumelli C, Raiteri L, Coco S, Paluzzi S, Caccin P, Rossetto O, Bonanno G, Montecucco C, Matteoli M. (literal)

Pagina inizio

• 142 (literal)

Pagina fine

• 153 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Traffic (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Univ Milan, Dept Med Pharmacol, I-20129 Milan, Italy 2. CNR, Inst Neurosci, Ctr Excellence Neurodegenerat Dis, I-20129 Milan, Italy 3. Univ Padua, Dipartimento Sci Biomed Sperimentali, I-35100 Padua, Italy 4. Univ Genoa, Ctr Excellence Biomed Res, Dept Expt Med, I-16100 Genoa, Italy (literal)

Titolo

• Traffic of botulinum toxins A and E in excitatory and inhibitory neurons. (literal)

Abstract

• Botulinum neurotoxins (BoNTs), proteases specific for the SNARE proteins, are used to study the molecular machinery supporting exocytosis and are used to treat human diseases characterized by cholinergic hyperactivity. The recent extension of the use of BoNTs to central nervous system (CNS) pathologies prompted the study of their traffic in central neurons. We used fluorescent BoNT/A and BoNT/E to study the penetration, the translocation and the catalytic action of these toxins in excitatory and inhibitory neurons. We show that BoNT/A and BoNT/E, besides preferentially inhibiting synaptic vesicle recycling at glutamatergic relative to Gamma-aminobutyric acid (GABA)-ergic neurons, are more efficient in impairing the release of excitatory than inhibitory neurotransmitter from brain synaptosomes. This differential effect does not result from a defective penetration of the toxin in line with the presence of the BoNT/A receptor, synaptic vesicle protein 2 (SV2), in both types of neurons. Interestingly, exogenous expression of SNAP-25 in GABAergic neurons confers sensitivity to BoNT/A. These results indicate that the expression of the toxin substrate, and not the toxin penetration, most likely accounts for the distinct effects of the two neurotoxins at the two types of terminals and support the use of BoNTs for the therapy of CNS diseases caused by the altered activity of selected neuronal populations. (literal)

Prodotto di

• Istituto di neuroscienze (IN) (Istituto)
• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)
• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)

Autore CNR

• CESARE MONTECUCCO (Persona)
• MICHELA MATTEOLI (Persona)
• CLAUDIA VERDERIO (Unità di personale interno)

Incoming links:

Autore CNR di

• CESARE MONTECUCCO (Persona)
• CLAUDIA VERDERIO (Unità di personale interno)
• MICHELA MATTEOLI (Persona)

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)
• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Traffic (Rivista)