The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• Galetto L., Bosco D., Balestrini R., Genre A., Fletcher J., Marzachì C. (2011)
  The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Galetto L., Bosco D., Balestrini R., Genre A., Fletcher J., Marzachì C. (literal)

Pagina inizio

• 1 (literal)

Pagina fine

• 12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 6(7) (literal)

Rivista

• PloS one (Rivista)

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• volume: 6(7): e22571 doi:10.1371/journal.pone.0022571 IMPACT FACTOR 4.411 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1) Galetto L., Marzachì C; 2) Bosco D.; 3) Genre A.; 4) Fletcher J. 1) Istituto di Virologia Vegetale, Consiglio Nazionale delle Ricerche, Torino, Italy, 2) Dipartimento di Valorizzazione e Protezione delle Risorse Agroalimentari - Entomologia e Zoologia Applicate all’Ambiente, Universita` degli Studi di Torino, Grugliasco, Italy, 3) Dipartimento di Biologia Vegetale, Universita` degli Studi di Torino, Torino, Italy, 4) Entomology and Plant Pathology Department, Oklahoma State University, Stillwater, Oklahoma, United States of America (literal)

Titolo

• The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors (literal)

Abstract

• Phytoplasmas, uncultivable phloem-limited phytopathogenic wall-less bacteria, represent a major threat to agriculture worldwide. They are transmitted in a persistent, propagative manner by phloem-sucking Hemipteran insects. Phytoplasma membrane proteins are in direct contact with hosts and are presumably involved in determining vector specificity. Such a role has been proposed for phytoplasma transmembrane proteins encoded by circular extrachromosomal elements, at least one of which is a plasmid. Little is known about the interactions between major phytoplasma antigenic membrane protein (Amp) and insect vector proteins. The aims of our work were to identify vector proteins interacting with Amp and to investigate their role in transmission specificity. In controlled transmission experiments, four Hemipteran species were identified as vectors of ‘‘Candidatus Phytoplasma asteris’’, the chrysanthemum yellows phytoplasmas (CYP) strain, and three others as non-vectors. Interactions between a labelled (recombinant) CYP Amp and insect proteins were analysed by far Western blots and affinity chromatography. Amp interacted specifically with a few proteins from vector species only. Among Amp-binding vector proteins, actin and both the a and b subunits of ATP synthase were identified by mass spectrometry and Western blots. Immunofluorescence confocal microscopy and Western blots of plasma membrane and mitochondrial fractions confirmed the localisation of ATP synthase, generally known as a mitochondrial protein, in plasma membranes of midgut and salivary gland cells in the vector Euscelidius variegatus. The vector-specific interaction between phytoplasma Amp and insect ATP synthase is demonstrated for the first time, and this work also supports the hypothesis that host actin is involved in the internalization and intracellular motility of phytoplasmas within their vectors. Phytoplasma Amp is hypothesized to play a crucial role in insect transmission specificity. (literal)

Prodotto di

• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)
• Istituto per la protezione delle piante (IPP) (Istituto)
• Biodiversità in sistemi agrari e forestali: basi genetiche, epigenetiche e molecolari (AG.P04.025.001) (Modulo)

Autore CNR

• RAFFAELLA MARIA BALESTRINI (Unità di personale interno)
• Luciana GALETTO (Persona)
• CRISTINA MARZACHI' (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• RAFFAELLA MARIA BALESTRINI (Unità di personale interno)
• CRISTINA MARZACHI' (Unità di personale interno)
• Luciana GALETTO (Persona)

Prodotto

• Istituto per la protezione delle piante (IPP) (Istituto)
• Resistenze convenzionali e non-convenzionali a importanti virosi e fitoplasmosi di specie coltivate (AG.P01.014.001) (Modulo)
• Biodiversità in sistemi agrari e forestali: basi genetiche, epigenetiche e molecolari (AG.P04.025.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)

Insieme di parole chiave di

• Keywords of "The Major Antigenic Membrane Protein of ‘‘Candidatus Phytoplasma asteris’’ Selectively Interacts with ATP Synthase and Actin of Leafhopper Vectors" (Insieme di parole chiave)