http://www.cnr.it/ontology/cnr/individuo/prodotto/ID172045

TSH induces co-localization of TSH receptor and Na/K-ATPase in human erythrocytes (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

TSH induces co-localization of TSH receptor and Na/K-ATPase in human erythrocytes (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1002/cbf.1567 (literal)

Alternative label

Balzan S.; Del Carratore R.; Nicolini G.; Forini F.; Lubrano V.; Simili M.; Benedetti P. A.; Iervasi G. (2009)
TSH induces co-localization of TSH receptor and Na/K-ATPase in human erythrocytes
in Cell biochemistry and function
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Balzan S.; Del Carratore R.; Nicolini G.; Forini F.; Lubrano V.; Simili M.; Benedetti P. A.; Iervasi G. (literal)

Pagina inizio

259 (literal)

Pagina fine

263 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

27 (literal)

Rivista

Cell biochemistry and function (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

In: Cell Biochemistry and Function, vol. 27 pp. 259 - 263. Wiley InterScience, 2009. (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

CNR-IFC, Pisa, CNR-IPCF, Pisa (literal)

Titolo

TSH induces co-localization of TSH receptor and Na/K-ATPase in human erythrocytes (literal)

Abstract

Thyroid stimulating hormone (TSH) binds to a specific TSH receptor (TSHR) which activates adenylate cyclase and increases cAMP levels in thyroidal cells. Recent studies have reported the presence of TSH receptor in several extra-thyroidal cell types, including erythrocytes. We have previously suggested that TSH is able to influence the erythrocyte Na/K-ATPase ouabain binding properties through a receptor mediated mechanism. The direct interaction of TSH receptor with the Na/K-pump and a functional role of TSHR in erythrocytes was not demonstrated. The interaction of TSH receptor with Na/K-pump and a TSHR functional role are not yet demonstrated in erythrocytes. In this study, we examined the interaction between the two receptors after TSH treatment using immunofluorescence coupled to confocal microscopy and a coimmunoprecipitation technique. The cAMP dependent signalling after TSH treatment was measured to verify TSHR functionality.We found that TSH receptor and Na/K-ATPase are localized on the membranes of both erythrocytes and erythrocyte ghosts; TSH receptor responds to TSH treatment by increasing intracellular cAMP levels from two to tenfold. In ghost membranes TSH treatment enhances up to three fold colocalization of TSHR with Na/K-ATPase and co-immunoprecipitation confirms their direct physical interaction. In conclusion our results are compatible with the existence, in erythrocytes, of a functional TSHR that interacts with Na/K-ATPase after TSH treatment, thus suggesting a novel cell signalling pathway, potentially active in local circulatory control. (literal)

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