http://www.cnr.it/ontology/cnr/individuo/prodotto/ID17204
Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations (Articolo in rivista)
- Type
- Label
- Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1080/10242420410001697089 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Pasta P.(3); Verga R.(1); Zambianchi F.(3); Daminati M.(2) (literal)
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- http://informahealthcare.com/doi/abs/10.1080/10242420410001697089 (literal)
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- Rivista
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- (1) Antibioticos S.p.A. - Research and Development; (2) Resindion SrL - Mitsubishi Chemical Corporation; (3) Ist. di Chim. Riconoscimento Molec. - CNR (literal)
- Titolo
- Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations (literal)
- Abstract
- Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of ?-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with >=98% conversion and 91-93% product yield. © 2004 Taylor & Francis Ltd. (literal)
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