Gene expression Profiling of embryonic human neural stem cells and dopaminergic neurons from adult human substantia nigra (Articolo in rivista)

Type
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  • Gene expression Profiling of embryonic human neural stem cells and dopaminergic neurons from adult human substantia nigra (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Hany E. S. Marei 1; Asma Althani 2; Nahla Afifi 2, Fabrizio Michetti 3; Mario Pescatori 3; Roberto Pallini 4; Patrizia Casalbore 5; Carlo Cenciarelli 6; Philip Schwartz 7; Abd-Elmaksoud Ahmed 1 (2011)
    Gene expression Profiling of embryonic human neural stem cells and dopaminergic neurons from adult human substantia nigra
    in PloS one
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Hany E. S. Marei 1; Asma Althani 2; Nahla Afifi 2, Fabrizio Michetti 3; Mario Pescatori 3; Roberto Pallini 4; Patrizia Casalbore 5; Carlo Cenciarelli 6; Philip Schwartz 7; Abd-Elmaksoud Ahmed 1 (literal)
Pagina inizio
  • e28420 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Epub 2011 Dec 7. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6(12) (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 - Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt; 2 - Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar; 3 - Institute of Anatomy and Cell Biology, Università Cattolica del S. Cuore, Roma, Italy; 4 - Institute of Neurosurgery, Università Cattolica del Sacro Cuore, Roma, Italy; 5 -Institute of Cell Biology and Neurobiology, National Research Council of Italy, Monterotondo Scalo, Rome, Italy; - 6 Institute of Translational Pharmacology, National Research Council of Italy, Rome, Italy; 7 - National Human Neural Stem Cell Resource, Children's Hospital of Orange County Research Institute, Orange, California, United States of America (literal)
Titolo
  • Gene expression Profiling of embryonic human neural stem cells and dopaminergic neurons from adult human substantia nigra (literal)
Abstract
  • Neural stem cells (NSC) with self-renewal and multipotent properties serve as an ideal cell source for transplantation to treat neurodegenerative insults such as Parkinson's disease. We used Agilent's and Illumina Whole Human Genome Oligonucleotide Microarray to compare the genomic profiles of human embryonic NSC at a single time point in culture, and a multicellular tissue from postmortem adult substantia nigra (SN) which are rich in dopaminergic (DA) neurons. We identified 13525 up-regulated genes in both cell types of which 3737 (27.6%) genes were up-regulated in the hENSC, 4116 (30.4%) genes were up-regulated in the human substantia nigra dopaminergic cells, and 5672 (41.93%) were significantly up-regulated in both cell population. Careful analysis of the data that emerged using DAVID has permitted us to distinguish several genes and pathways that are involved in dopaminergic (DA) differentiation, and to identify the crucial signaling pathways that direct the process of differentiation. The set of genes expressed more highly at hENSC is enriched in molecules known or predicted to be involved in the M phase of the mitotic cell cycle. On the other hand, the genes enriched in SN cells include a different set of functional categories, namely synaptic transmission, central nervous system development, structural constituents of the myelin sheath, the internode region of axons, myelination, cell projection, cell somata, ion transport, and the voltage-gated ion channel complex. Our results were also compared with data from various databases, and between different types of arrays, Agilent versus Illumina. This approach has allowed us to confirm the consistency of our obtained results for a large number of genes that delineate the phenotypical differences of embryonic NSCs, and SN cells. (literal)
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