Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00249-010-0607-x (literal)

Alternative label

• Bramanti, Emilia (2); Lenci, Francesco (1); Sgarbossa, Antonella (1) (2010)
  Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides
  in European biophysics journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bramanti, Emilia (2); Lenci, Francesco (1); Sgarbossa, Antonella (1) (literal)

Pagina inizio

• 1493 (literal)

Pagina fine

• 1501 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://puma.isti.cnr.it/linkres.php?resource=cnr.ibf/cnr.ibf.pi/2010-A0-006 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 39 (literal)

Rivista

• European biophysics journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) CNR-IBF, Pisa; (2) CNR-IPCF, Pisa (literal)

Titolo

• Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides (literal)

Abstract

• We have determined the secondary structure of 1-40 beta-amyloid peptides by Fourier-transform infrared spectroscopy (FTIR) and characterized the peptide photophysical properties before and after self-assembly by using intrinsic tyrosine steady-state and time-resolved fluorescence. All measurements were performed in the presence and absence of hypericin (Hyp), an exogenous natural polycyclic pigment that has been shown to inhibit fibril formation and has also been used as a fluorescent probe. We monitored the time course of the aggregation process measuring 405 nm light diffusion at 90° and used thioflavin T to reveal the presence of fibrils. FTIR quantitative analysis evidenced a prevalent random conformation at t = 0 with and without Hyp. Fibrils showed a predominant parallel beta-sheet structure and a small percentage of alpha-helix. The results of fluorescence measurements showed that Hyp does significantly interact with peptides in beta-sheet conformation. In conclusion, hypericin does hinder the formation of fibrils, but the percentages of parallel beta-sheets were not significantly different from those found in samples not treated with Hyp. (literal)

Prodotto di

• Istituto di biofisica (IBF) (Istituto)
• Istituto per i processi chimico-fisici (IPCF) (Istituto)
• Processi Fotoindotti in Biomolecole e Cellule (MD.P01.003.001) (Modulo)
• Chimica analitica strumentale (PM.P03.013.005) (Modulo)

Autore CNR

• ANTONELLA SGARBOSSA (Unità di personale interno)
• EMILIA BRAMANTI (Persona)
• FRANCESCO LENCI (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per i processi chimico-fisici (IPCF) (Istituto)
• Istituto di biofisica (IBF) (Istituto)
• Chimica analitica strumentale (PM.P03.013.005) (Modulo)
• Processi Fotoindotti in Biomolecole e Cellule (MD.P01.003.001) (Modulo)

Autore CNR di

• ANTONELLA SGARBOSSA (Unità di personale interno)
• EMILIA BRAMANTI (Persona)
• FRANCESCO LENCI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European biophysics journal (Rivista)

Insieme di parole chiave di

• Keywords of "Effects of hypericin on the structure and aggregation properties of beta-amyloid peptides" (Insieme di parole chiave)