Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L. genotypes (Articolo in rivista)

Type
Label
  • Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L. genotypes (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/treephys/tpr083 (literal)
Alternative label
  • Beritognolo I., Harfouche A., Brilli F., Prosperini G., Gaudet M., Brosché M., Salani F., Kuzminsky E., Auvinen P., Paulin L., Kangasjärvi J., Loreto F., Valentini R., Scarascia Mugnozza G., Sabatti M. (2011)
    Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L. genotypes
    in Tree physiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Beritognolo I., Harfouche A., Brilli F., Prosperini G., Gaudet M., Brosché M., Salani F., Kuzminsky E., Auvinen P., Paulin L., Kangasjärvi J., Loreto F., Valentini R., Scarascia Mugnozza G., Sabatti M. (literal)
Pagina inizio
  • 1335 (literal)
Pagina fine
  • 1355 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Settore Scientifico Disciplinare AGR/07 genetica agraria (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 31 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • First published online September 12, 2011 doi:10.1093/treephys/tpr083. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy 2) Institute for Agriculture and Forest Systems in the Mediterranean (CNR-ISAFOM), National Research Council, Via Madonna Alta 128, 06128 Perugia, Italy 3) Institute of Agroenvironmental and Forest Biology (CNR-IBAF), National Research Council, Via Salaria Km 29,300, 00015 Monterotondo Scalo, Roma, Italy; 4) CASPUR, Consorzio interuniversitario per le applicazioni di supercalcolo per università e ricerca, Via dei Tizii 6, 00185, Roma, Italy 5) Department of Biosciences, Division of Plant Biology, University of Helsinki, Viikinkaari 1 (PL 65) 00014 Helsinki, Finland; 6) Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland 7) Plant Protection Institute (CNR-IPP), National Research Council, Via Madonna del Piano, 10 50019 Sesto Fiorentino, Firenze, Italy; 8) Department of Agronomy, Forestry and Land Use (DAF), Agricultural Research Council (CRA), Via del Caravita 7/a, 00186 Roma, Italy; (literal)
Titolo
  • Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L. genotypes (literal)
Abstract
  • Soil salinity is an important limiting factor to tree growth and productivity. Populus alba L. is a moderately salt-tolerant species and its natural populations are adapted to contrasting environments, thus providing genetic resources to identify key genes for tolerance to abiotic stress, such as salinity. To elucidate the molecular and genetic basis of variation for salinity tolerance in P. alba, we analyzed the short-term ecophysiological and transcriptome response to salinity. Two contrasting genotypes, 6K3, salt sensitive, and 14P11, salt tolerant, originating from North and South Italy, respectively, were challenged with salt stress (200 mM NaCl). Sodium accumulated in the leaves of salt-treated plants and its concentration increased with time. The net photosynthesis was strongly reduced by salinity in both genotypes, with 6K3 being significantly more affected than 14P11. The transcriptional changes in leaves were analyzed using cDNA microarrays containing about 7000 stressrelated poplar expressed sequence tags (EST). A microarray experiment based on RNA pooling showed a number of salinity-regulated transcripts that markedly increased from 3 h to 3 days of salinity treatment. Thus, a detailed analysis was performed on replicated plants collected at 3 days, when ~20% of transcripts showed significant change induced by salinity. In 6K3, there were more genes with decreased expression than genes with increased expression, whereas such a difference was not found in 14P11. Most transcripts with decreased expression were shared between the two genotypes, whereas transcripts with increased expression were mostly regulated in a genotype-specific manner. The commonly decreased transcripts (71 genes) were functionally related to carbohydrate metabolism, energy metabolism and photosynthesis. These biological processes were consistent with the strong inhibition of photosynthesis, caused by salinity. The commonly increased transcripts (13 genes) were functionally related to primary metabolism and biosynthesis of proteins and macromolecules. The salinityincreased transcripts discriminated the molecular response of the two genotypes. In 14P11, the 21 genes specifically salinityinduced were related to stress response, cell development, cell death and catabolism. In 6K3, the 15 genes with salinity-increased expression were involved in protein biosynthesis, metabolism of macromolecules and cell organization and biogenesis. The difference in transcriptome response between the two genotypes could address the molecular basis of intraspecific variation of salinity tolerance in P. alba. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it